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Related Experiment Video

Updated: May 21, 2026

Quantification of Cell-Substrate Adhesion Area and Cell Shape Distributions in MCF7 Cell Monolayers
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Quantification of Cell-Substrate Adhesion Area and Cell Shape Distributions in MCF7 Cell Monolayers

Published on: June 24, 2020

Three-dimensional cell morphometry for the quantification of cell-substrate interactions.

Michael Shaw1, Nilofar Faruqui, Elzbieta Gurdak

  • 1Analytical Science Division, National Physical Laboratory, London, United Kingdom. mike.shaw@npl.co.uk

Tissue Engineering. Part C, Methods
|June 19, 2012
PubMed
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Quantifying cell attachment to biomaterials is key for biocompatibility. New 3D cell morphometry methods reveal fibronectin and titanium coatings enhance osteosarcoma cell spreading on substrates.

Area of Science:

  • Biomaterials Science
  • Cell Biology
  • Surface Chemistry

Background:

  • Initial cell attachment to biomaterials predicts long-term biocompatibility.
  • Quantifying cell-substrate interactions is crucial for biomaterial development and quality control.

Purpose of the Study:

  • To develop and validate a protocol for measuring temporal changes in mammalian cell 3D morphology on various substrates.
  • To assess the impact of fibronectin coating and titanium on osteosarcoma cell spreading and morphology.

Main Methods:

  • Utilized fluorescence confocal laser scanning microscopy and image processing.
  • Measured temporal changes in cell thickness, volume, and footprint area.
  • Investigated osteosarcoma cells on uncoated glass, fibronectin-coated glass, and titanium substrates.

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Published on: November 29, 2017

An Analytical Tool that Quantifies Cellular Morphology Changes from Three-dimensional Fluorescence Images
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An Analytical Tool that Quantifies Cellular Morphology Changes from Three-dimensional Fluorescence Images

Published on: August 31, 2012

Related Experiment Videos

Last Updated: May 21, 2026

Quantification of Cell-Substrate Adhesion Area and Cell Shape Distributions in MCF7 Cell Monolayers
06:46

Quantification of Cell-Substrate Adhesion Area and Cell Shape Distributions in MCF7 Cell Monolayers

Published on: June 24, 2020

Mammalian Cell Division in 3D Matrices via Quantitative Confocal Reflection Microscopy
10:22

Mammalian Cell Division in 3D Matrices via Quantitative Confocal Reflection Microscopy

Published on: November 29, 2017

An Analytical Tool that Quantifies Cellular Morphology Changes from Three-dimensional Fluorescence Images
10:00

An Analytical Tool that Quantifies Cellular Morphology Changes from Three-dimensional Fluorescence Images

Published on: August 31, 2012

Main Results:

  • Fibronectin coating significantly increased cell spreading, indicated by larger footprint area and decreased thickness.
  • Osteosarcoma cells exhibited faster spreading on titanium compared to uncoated glass.
  • 3D cell morphometry provided quantitative data on initial cell-substrate interactions.

Conclusions:

  • 3D cell morphometry is a valuable multiparametric tool for assessing initial cell-substrate interactions.
  • Findings demonstrate enhanced biocompatibility with fibronectin coatings and titanium substrates.
  • The developed protocol has applications in quality control and tailored surface finish development.