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Published on: September 19, 2017
Development of a sandwich format, amperometric screen-printed uric acid biosensor for urine analysis
P Kanyong1, R M Pemberton, S K Jackson
1Centre for Research in Biosciences, University of the West of England, Frenchay Campus, Bristol BS16 1QY, UK.
Analytical Biochemistry
|June 19, 2012
Summary
A novel uric acid biosensor utilizing a cobalt phthalocyanine-modified electrode demonstrates high precision and recovery for urine analysis. This electrochemical sensor offers a sensitive and reliable method for detecting uric acid levels.
Area of Science:
- Electrochemistry
- Biosensor Technology
- Analytical Chemistry
Background:
- Uric acid levels are critical indicators of various health conditions.
- Development of sensitive and selective biosensors is crucial for accurate uric acid monitoring.
- Screen-printed carbon electrodes (SPCEs) offer a cost-effective and versatile platform for biosensor fabrication.
Purpose of the Study:
- To develop and optimize a novel uric acid biosensor.
- To investigate the performance of a cobalt phthalocyanine (CoPC) modified SPCE as a transducer.
- To evaluate the biosensor's efficacy in analyzing uric acid in human urine samples.
Main Methods:
- Fabrication of a sandwich biosensor (PC-UOX-CA-CoPC-SPCE) using a water-based ink formulation.
- Optimization of chronoamperometric analysis conditions including temperature, pH, ionic strength, enzyme loading, and incubation time.
- Application of the biosensor to analyze uric acid in real human urine samples.
Main Results:
- Optimized conditions: 35°C, pH 9.2, 50 mM buffer, 0.6 U uricase, 180 s incubation.
- High precision on unspiked urine (5.82%) and fortified urine (4.21%).
- Excellent recovery (97.3%) and a linear working range of 0.015 to 0.25 mM with a detection limit of 0.015 mM.
- Sensitivity of 2.10 μA/mM.
Conclusions:
- The developed uric acid biosensor demonstrates excellent analytical performance.
- The CoPC-modified SPCE is a suitable transducer for sensitive uric acid detection.
- The biosensor shows potential for reliable and accurate monitoring of uric acid in clinical urine samples.
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