Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

[Coating Technology for Flow Diverter Devices].

No shinkei geka. Neurological surgery·2026

Same author

Analysis of site-dependent mucins in rat intestinal mucosa using anti-glycan monoclonal antibodies.

Glycoconjugate journal·2025

Same author

Advances in bioinspired polymer hydrogel systems with biomedical functionalities.

Science and technology of advanced materials·2025

Same author

Biomimetic polymers with phosphorylcholine groups as biomaterials for medical devices.

Proceedings of the Japan Academy. Series B, Physical and biological sciences·2024

Same author

Osteogenic tailoring of oriented bone matrix organization using on/off micropatterning for osteoblast adhesion on titanium surfaces.

Acta biomaterialia·2024

Same author

Antithrombotic Revascularization Strategy of Bioengineered Liver Using a Biomimetic Polymer.

Tissue engineering. Part A·2024

Search research articles

Related Experiment Video

Updated: May 21, 2026

Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays

Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays

Published on: March 9, 2017

Electrospun phospholipid polymer substrate for enhanced performance in immunoassay system.

Surasak Chantasirichot¹, Kazuhiko Ishihara

¹Department of Materials Engineering, School of Engineering, The University of Tokyo, Tokyo 113-8656, Japan.

Biosensors & Bioelectronics

|June 19, 2012

Summary

A novel polymer, poly[2-methacryloyloxyethyl phosphorylcholine (MPC)-co-n-butyl methacrylate (BMA)-co-N-succinimidyloxycarbonyl di(ethylene glycol) methacrylate (PENHS)] (PMBS), enhances immunoassays. Its nanostructure reduces background noise and improves sensitivity for biosensing applications.

More Related Videos

Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood

Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood

Published on: April 16, 2016

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay

Published on: February 23, 2020

Related Experiment Videos

Last Updated: May 21, 2026

Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays

Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays

Published on: March 9, 2017

Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood

Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood

Published on: April 16, 2016

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay

Published on: February 23, 2020

Area of Science:

Polymer Chemistry
Biomaterials Science
Biosensor Technology

Background:

Immunoassays often suffer from background noise due to nonspecific protein adsorption.
Conventional substrates like polystyrene can lead to high background, reducing assay specificity and signal-to-noise ratio.
Developing biomolecule-friendly platforms is crucial for improving immunoassay performance.

Purpose of the Study:

To develop a functional polymer platform for enhanced immunoassays.
To utilize electrospinning for creating a nanostructured polymer with improved biomolecule compatibility.
To evaluate the performance of the nanostructured polymer in enzyme-linked immunosorbent assays (ELISA).

Main Methods:

Synthesis of poly[2-methacryloyloxyethyl phosphorylcholine (MPC)-co-n-butyl methacrylate (BMA)-co-N-succinimidyloxycarbonyl di(ethylene glycol) methacrylate (PENHS)] (PMBS).
Fabrication of nanofibrous PMBS using electrospinning deposition.
Evaluation of PMBS performance in ELISA for human immunoglobulin-G, comparing it with polystyrene substrates.

Main Results:

The nonbiofouling PMBS significantly reduced background noise and nonspecific adsorption in ELISA.
Electrospun PMBS nanostructures increased surface area, enhancing antibody binding and biosensor sensitivity.
The PMBS substrate demonstrated a linear detection range of 1.0–100 ng/mL for human IgG and shortened detection time by 25% by omitting the blocking step.
Immobilized antibodies retained significant bioactivity after 4 weeks of dry storage.

Conclusions:

Nanostructured PMBS provides a highly biomolecule-friendly platform for immunoassays.
PMBS enhances immunoassay reliability, signal-to-noise ratio, and sensitivity.
The developed material improves the stability and lifetime of immobilized biomolecules for biosensor applications.