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In vitro effect of plasma during phenylhydrazine-induced erythrocyte oxidative damage
F J Lucio Cazana1, M L Diez Marques, A Jimenez
1Department of Physiology and Pharmacology, University of Alcala de Henares, Madrid, Spain.
Biomedica Biochimica Acta
|January 1, 1990
Summary
Plasma exacerbates oxidant-induced erythrocyte damage by increasing lipid peroxidation and reducing Na+ extrusion, despite maintaining membrane fluidity. This suggests plasma actively contributes to oxidative hemolysis in vivo.
Area of Science:
- Hematology
- Oxidative Stress
- Cellular Physiology
Background:
- Oxidative stress is a significant factor in erythrocyte damage and hemolysis.
- The role of plasma in modulating erythrocyte response to oxidants is not fully understood.
Purpose of the Study:
- To investigate the effect of plasma on erythrocyte damage induced by phenylhydrazine hydrochloride.
- To elucidate the mechanisms by which plasma influences erythrocyte susceptibility to oxidative damage.
Main Methods:
- Erythrocytes were incubated in plasma or phosphate-buffered saline with phenylhydrazine hydrochloride.
- Measurements included membrane fluidity, erythrocyte filterability, active sodium extrusion, and lipid peroxidation.
Main Results:
- Plasma prevented the decrease in membrane fluidity but increased erythrocyte lipid peroxidation by 100%.
- Erythrocytes in plasma showed significantly lower filterability and active Na+ extrusion compared to those in plasma-phenylhydrazine.
- Phenylhydrazine hydrochloride induced significant erythrocyte damage.
Conclusions:
- Plasma plays an active role in oxidant-induced erythrocyte damage.
- Plasma contributes to hemolytic effects of oxidation through mechanisms beyond membrane fluidity.
- These findings highlight plasma's complex role in vivo during oxidative stress.