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Updated: May 21, 2026
In Vivo Two-Color 2-Photon Imaging of Genetically-Tagged Reporter Cells in the Skin
Published on: July 11, 2019
Microarray-guided discovery of two-photon (2P) small molecule probes for live-cell imaging of cysteinyl cathepsin
Zhenkun Na1, Lin Li, Mahesh Uttamchandani
1Department of Chemistry, National University of Singapore, 3 Science Drive 3, 117543, Singapore.
Abstract:
A microarray immobilized with 105 aldehyde-containing small molecules was screened against mammalian cell lysates over-expressing cathepsin L to identify two potent inhibitors, which were subsequently converted into cell-permeable probes capable of live-cell imaging of endogenous cysteinyl cathepsin activities by two-photon fluorescence microscopy.
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