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Related Concept Videos

Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Ribosomes01:27

Ribosomes

Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within...
Ribosomes01:27

Ribosomes

Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within...
Ribosomes01:27

Ribosomes

Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...

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Single Molecule Fluorescence Energy Transfer Study of Ribosome Protein Synthesis
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Published on: July 6, 2021

RNA-magnesium-protein interactions in large ribosomal subunit.

Anton S Petrov1, Chad R Bernier, Chiaolong Hsiao

  • 1School of Biology, Georgia Institute of Technology, Atlanta, Georgia 30332, United States.

The Journal of Physical Chemistry. B
|June 21, 2012
PubMed
Summary

Magnesium ions in ribosomes link RNA sequences and interact with proteins like L2. This study reveals magnesium

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Structural Biology

Background:

  • Magnesium ions are crucial for ribosome structure and function.
  • Magnesium ions stabilize ribosomal RNA (rRNA) through coordination with phosphate groups.
  • Interactions between magnesium, rRNA, and ribosomal proteins are not fully understood.

Purpose of the Study:

  • To investigate the specific roles of magnesium ions in stabilizing ribosomal structures.
  • To elucidate the magnesium-mediated interactions between rRNA and ribosomal protein L2.
  • To quantify the energetic contributions of magnesium in these interactions.

Main Methods:

  • Quantum mechanical analysis using density functional theory (DFT).
  • Natural energy decomposition analysis (NEDA) on optimized models from X-ray structures.
  • Modeling of rRNA/magnesium/water/rProtein L2 assemblies.

Main Results:

  • RNA immobilizes magnesium ions via multidentate chelation with phosphate groups.
  • Magnesium ions localize and polarize water molecules, enhancing their interaction with ribosomal protein L2.
  • Magnesium ions play specific roles beyond electrostatic neutralization and direct RNA coordination.

Conclusions:

  • Magnesium ions are essential for specific magnesium-mediated interactions involving ribosomal protein L2.
  • Magnesium ions contribute to ribosomal assembly by influencing water molecule interactions with proteins.
  • The findings highlight subtle but significant roles of magnesium in ribosome structure and function.