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Related Concept Videos

Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Protein Complex Assembly02:41

Protein Complex Assembly

Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Protein Complex Assembly02:41

Protein Complex Assembly

Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Additional Subnuclear Structures02:10

Additional Subnuclear Structures

The eukaryotic nucleus is a double membrane-bound organelle that contains nearly all of the cell’s genetic material in the form of chromosomes. It is rightly called the “brain” of the cell as it shoulders the responsibility of responding to various physiological processes, stress, altered metabolic conditions, and other cellular signals. 
The nucleus contains many membrane-less subnuclear organelles or nuclear bodies, such as nucleoli, Cajal bodies, speckles, paraspeckles, etc. These nuclear...
Additional Subnuclear Structures02:10

Additional Subnuclear Structures

The eukaryotic nucleus is a double membrane-bound organelle that contains nearly all of the cell’s genetic material in the form of chromosomes. It is rightly called the “brain” of the cell as it shoulders the responsibility of responding to various physiological processes, stress, altered metabolic conditions, and other cellular signals. 
The nucleus contains many membrane-less subnuclear organelles or nuclear bodies, such as nucleoli, Cajal bodies, speckles, paraspeckles, etc. These nuclear...

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Updated: May 21, 2026

High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis
09:33

High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis

Published on: October 15, 2019

Dissecting the NUP107 complex: multiple components and even more functions.

Cristina González-Aguilera1, Peter Askjaer

  • 1Centro Andaluz de Biología del Desarrollo (CABD), Consejo Superior de Investigaciones Científicas, Universidad Pablo de Olavide, Seville, Spain.

Nucleus (Austin, Tex.)
|June 21, 2012
PubMed
Summary
This summary is machine-generated.

The NUP107 complex, crucial for Nuclear Pore Complex (NPC) assembly and function, has revealed new insights into its own structure and a surprising link to the MAD1 mitotic checkpoint protein.

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Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling
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Related Experiment Videos

Last Updated: May 21, 2026

High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis
09:33

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Published on: October 15, 2019

Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling
09:35

Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling

Published on: April 1, 2017

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Structural Biology

Background:

  • The Nuclear Pore Complex (NPC) is a vital cellular gatekeeper.
  • The NUP107 complex is a key NPC subcomplex with diverse roles.
  • Understanding NPC subcomplexes is crucial for cell function.

Purpose of the Study:

  • To investigate the assembly and function of the NUP107 complex.
  • To explore novel interactions and roles of the NUP107 complex.
  • To elucidate the connection between NUP107 complex and cell division.

Main Methods:

  • Genetic dissection of the NUP107 complex.
  • Analysis of NPC assembly and function.
  • Investigating interactions with cell cycle proteins.

Main Results:

  • Novel insights into the assembly pathway of the NUP107 complex.
  • Identification of an unexpected link between the NUP107 complex and MAD1.
  • MAD1 is implicated in NUP107 complex-related functions.

Conclusions:

  • The NUP107 complex plays a multifaceted role beyond NPC formation.
  • The NUP107 complex has a previously unrecognized connection to the mitotic spindle assembly checkpoint.
  • Further research on the NUP107 complex and MAD1 interaction is warranted.