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Updated: May 21, 2026
Studying DNA Looping by Single-Molecule FRET
Published on: June 28, 2014
Panorama of DNA hairpin folding observed via diffusion-decelerated fluorescence correlation spectroscopy
Yandong Yin1, Peng Wang1, Xin Xing Yang1
1Beijing National Laboratory for Molecular Sciences and Department of Chemical Biology, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China. zhaoxs@pku.edu.cn and State Key Laboratory for Structural Chemistry of Unstable and Stable Species and Biodynamic Optical Imaging Centre, Peking University, Beijing 100871, China.
Abstract:
Based on a confocal microscopy platform, we extended the FCS time window by three orders of magnitude to the s timescale by attaching a polystyrene microsphere. We simultaneously monitored the relaxations of multiple intermediates involved in DNA hairpin folding, thus offering a much more detailed view of the kinetics of hairpin folding experimentally.
