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Updated: May 21, 2026
08:20
In Vitro Differentiation of Human CD4+FOXP3+ Induced Regulatory T Cells (iTregs) from Naïve CD4+ T Cells Using a TGF-β-containing Protocol
Published on: December 30, 2016
FOXO3: A master switch for regulating tolerance and immunity in dendritic cells.
Stephanie K Watkins1, Arthur A Hurwitz
1Tumor Immunity and Tolerance Section; Laboratory of Molecular Immunoregulation; Cancer and Inflammation Program; NCI-Frederick; Frederick, MD USA.
Oncoimmunology
|June 22, 2012
Summary
Prostate tumors use dendritic cells to suppress CD8(+) T cell responses. Silencing Foxo3 in these cells enhances anti-tumor immunity, making FOXO3 a potential immunotherapy target.
Area of Science:
- Immunology
- Oncology
- Molecular Biology
Background:
- Dendritic cells (DCs) within prostate tumors are known to induce immune tolerance.
- This tolerance specifically affects tumor antigen-specific CD8(+) T cells, hindering anti-tumor immune responses.
Purpose of the Study:
- To investigate the role of Foxo3 expression in regulating DC tolerogenicity in prostate tumors.
- To determine if modulating Foxo3 expression can enhance anti-tumor immunity.
Main Methods:
- Analysis of Foxo3 expression in tumor-infiltrating dendritic cells.
- Experimental manipulation of Foxo3 expression in DCs.
- Assessment of CD8(+) T cell responses against tumor antigens.
Main Results:
- Foxo3 expression in dendritic cells correlates with immune tolerance in prostate tumors.
- Silencing Foxo3 expression in DCs reverses immune tolerance.
- Enhanced anti-tumor immune responses were observed following Foxo3 silencing.
Conclusions:
- Dendritic cell tolerogenicity in prostate tumors is regulated by Foxo3 expression.
- Targeting FOXO3 by silencing its expression represents a potential strategy for prostate cancer immunotherapy.
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