Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Eight-parameter PC-AT based flow cytometric data system.

V Kachel1, R Messerschmidt, P Hummel

  • 1Max-Planck-Institut für Biochemie, Martinsried, Federal Republic of Germany.

Cytometry
|January 1, 1990
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

MODELING STREAMFLOW AND WATER QUALITY SENSITIVITY TO CLIMATE CHANGE AND URBAN DEVELOPMENT IN 20 U.S. WATERSHEDS.

Journal of the American Water Resources Association·2022
Same author

Ethical issues surrounding childhood tuberculosis.

The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease·2020
Same author

Should not the terms 'width', 'depth' and 'length' be used in accordance with anatomical planes?

Ultrasound in obstetrics & gynecology : the official journal of the International Society of Ultrasound in Obstetrics and Gynecology·2013
Same author

Effect of 17-alpha hydroxyprogesterone caproate on cervical length in twin pregnancies.

Ultrasound in obstetrics & gynecology : the official journal of the International Society of Ultrasound in Obstetrics and Gynecology·2012
Same author

Vitamin D and gestational diabetes: a systematic review and meta-analysis.

European journal of internal medicine·2012
Same author

Validity and reliability of the N-PASS assessment tool with acute pain.

Journal of perinatology : official journal of the California Perinatal Association·2009

A new 8-parameter flow cytometry system uses a personal computer and custom interface for data acquisition. This system offers automated trigger thresholds and versatile data processing modes for enhanced biological analysis.

Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Instrumentation

Background:

  • Flow cytometry is a powerful technique for analyzing single cells.
  • Existing systems may have limitations in parameter acquisition and data processing.
  • The need for cost-effective and versatile flow cytometric data systems is ongoing.

Purpose of the Study:

  • To describe a novel 8-parameter flow cytometric data system.
  • To detail the hardware and software components of the system.
  • To highlight the system's capabilities for data acquisition and analysis.

Main Methods:

  • Utilized an IBM-AT compatible personal computer (PC) with a commercial analog-to-digital conversion (ADC) board.
  • Developed a dedicated pulse processing interface for adapting flow cytometric pulses and controlling parameters.

Related Experiment Videos

  • Implemented software in assembly language for system operation and data processing.

    • Main Results:

    • The system supports 1-8 parameter listmode or 1-3 parameter histogram data collection.
    • Automated trigger thresholds are set above noise levels for improved signal detection.
    • Features include graphical color displays, numerical integration, multiparameter gating, and printing capabilities.

    Conclusions:

    • The described system provides a flexible and efficient platform for multi-parameter flow cytometry.
    • The integration of PC hardware and custom interface offers a cost-effective solution.
    • The system's functionalities facilitate advanced data analysis in biological research.