Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Molecular Factors Affecting Cell Division01:27

Molecular Factors Affecting Cell Division

Several external and internal factors influence the initiation and inhibition of cell division. For instance, the death of nearby cells or the release of human growth hormone (hGH) promotes cell division. In contrast, lack of hGH or crowding of cells can inhibit cell division.
Several proteins function as internal regulators to ensure each cell cycle stage is completed faithfully before proceeding to the next. Regulator molecules may act directly or influence the activity or production of other...
Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
The Cell Cycle Control System01:28

The Cell Cycle Control System

The cell cycle regulation directs how a cell proceeds from one phase to the next and begins mitosis. The cell cycle control system includes intracellular regulatory molecules and external triggers. They provide "stop" or "advance" signals and operate at specific cell cycle stages termed checkpoints to ensure that a particular process is completed before the cell advances to the next phase.
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and function at the cell...
The Cell Cycle Control System02:11

The Cell Cycle Control System

The cell cycle is an organized set of events that leads the cell to divide into two daughter cells, each containing chromosomes identical to the parent cell. It is the cell cycle that leads to the formation of an entire organism from a single-cell zygote. Besides, cell division also functions in the renewal or repair of tissues in adult multicellular eukaryotes. For example, in the bone marrow, the stem cells divide to form new blood cells. Although essential for several functions, cell...
The Cell Cycle Control System02:11

The Cell Cycle Control System

The cell cycle is an organized set of events that leads the cell to divide into two daughter cells, each containing chromosomes identical to the parent cell. It is the cell cycle that leads to the formation of an entire organism from a single-cell zygote. Besides, cell division also functions in the renewal or repair of tissues in adult multicellular eukaryotes. For example, in the bone marrow, the stem cells divide to form new blood cells. Although essential for several functions, cell...
Positive Regulator Molecules02:39

Positive Regulator Molecules

Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Understanding the Content and Purpose of S3 Clinical Practice Guidelines in Dentistry.

International endodontic journal·2026
Same author

Epigenetic regulation of cardiac physiology and pathophysiology: biological sex matters.

The journal of cardiovascular aging·2026
Same author

Pharmacokinetic and Pharmacodynamic Assessments of the Ivermectin and Levamisole Combination to Control Resistant Nematodes in Cattle.

Pharmaceutics·2026
Same author

In Vitro Effects of Combining Resin Infiltration and At-Home Bleaching on Hydrogen Peroxide Penetration, Color Change and Enamel Morphology.

Journal of esthetic and restorative dentistry : official publication of the American Academy of Esthetic Dentistry ... [et al.]·2026
Same author

CTCF Regulates Erythroid Differentiation Through Control of Core Erythroid Transcription Factors.

Biomolecules·2026
Same author

Patient satisfaction with at-home dental bleaching using Vivera or Essix retainers: a randomised clinical trial.

BMC oral health·2026

Related Experiment Video

Updated: May 21, 2026

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries
10:10

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

Published on: March 31, 2019

A cell cycle role for the epigenetic factor CTCF-L/BORIS.

Manuel Rosa-Garrido1, Laura Ceballos, Pilar Alonso-Lecue

  • 1Cell Cycle, Stem Cell Fate and Cancer Laboratory, Fundación Marqués de Valdecilla-Instituto de Formación e Investigación Marqués de Valdecilla, Santander, Spain.

Plos One
|June 23, 2012
PubMed
Summary

The protein BORIS, an antagonist of CTCF, accumulates during cell cycle arrest and DNA damage. Its downregulation inhibits transcription and cell cycle progression, suggesting a role in coordinating S phase and mitosis.

More Related Videos

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
12:02

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

Published on: June 6, 2017

In Vitro Differentiation Model of Human Normal Memory B Cells to Long-lived Plasma Cells
10:26

In Vitro Differentiation Model of Human Normal Memory B Cells to Long-lived Plasma Cells

Published on: January 20, 2019

Related Experiment Videos

Last Updated: May 21, 2026

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries
10:10

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

Published on: March 31, 2019

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
12:02

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

Published on: June 6, 2017

In Vitro Differentiation Model of Human Normal Memory B Cells to Long-lived Plasma Cells
10:26

In Vitro Differentiation Model of Human Normal Memory B Cells to Long-lived Plasma Cells

Published on: January 20, 2019

Area of Science:

  • Epigenetics
  • Cell Biology
  • Molecular Biology

Background:

  • CTCF is a key epigenetic regulator of chromatin organization.
  • BORIS (CTCF-like) antagonizes CTCF and is found in various cell types, with its function being intriguing.
  • BORIS may play a role in developmental reprogramming of gene expression.

Purpose of the Study:

  • To investigate the expression and function of CTCF and BORIS proteins in human epidermis.
  • To elucidate the role of BORIS in cellular processes like transcription and cell cycle progression.

Main Methods:

  • Immunofluorescence microscopy to determine protein localization (CTCF and BORIS).
  • Nascent RNA detection in primary keratinocytes.
  • Cell cycle analysis (S phase, mitosis) and DNA damage induction.
  • RNA interference (shRNA) to down-regulate BORIS expression.
  • Ectopic expression of BORIS.

Main Results:

  • CTCF is nuclear, while BORIS localizes to the nucleolus, euchromatin, and active transcription sites.
  • BORIS is also found at interphase centrosomes and accumulates upon cell cycle arrest (S phase, mitosis) or DNA damage.
  • Ectopic BORIS expression increases S phase cells and causes genomic instability.
  • BORIS down-regulation by shRNA inhibits RNA transcription and cell cycle progression.

Conclusions:

  • BORIS is involved in regulating gene transcription and cell cycle progression.
  • The findings suggest BORIS plays a role in coordinating S phase events with mitosis.
  • BORIS's localization to centrosomes and accumulation during cell cycle stress highlight its potential role in cell cycle regulation and genomic stability.