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Published on: July 30, 2020
[Identification of signal transduction pathway in fresh and vitrified porcine oocytes]
Abstract:
Signal transduction pathway under the influence of somatotropin have been identified basis on the analysis of Ca2+ release from intracellular stores of fresh and vitrified porcine oocytes using inhibitory analysis. Somatotropin and GTP individually stimulated Ca2+ release from intracellular stores. The joint action of somatotropin and GTP activated additional Ca2+ release from intracellular stores both in fresh and vitrified porcine oocytes. Treatment of the oocytes with inhibitor of protein kinase C caused no additional Ca2+ release from intracellular stores. Ca2+ release from intracellular stores stimulated by GTP was connected with phosphate hydrolysis. Moving between intracellular Ca2+ depots stimulated by GTP was not determined by phosphate hydrolysis. Inhibitor of protein kinase C and microtubules were involved in the interaction of various intracellular depots. The data obtained suggest that signal transduction pathway in porcine oocytes do not change after vitrification.
Insights
Vitrification does not alter the signal transduction pathway in porcine oocytes. Somatotropin and GTP stimulate calcium (Ca2+) release, indicating preserved cellular mechanisms after cryopreservation.
Area of Science:
- Reproductive biology
- Cellular signaling
- Cryopreservation
Background:
- Somatotropin plays a role in cellular processes.
- Calcium (Ca2+) signaling is crucial in oocyte function.
- Vitrification is a common method for preserving oocytes.
Purpose of the Study:
- To investigate the signal transduction pathway in porcine oocytes.
- To determine the effect of somatotropin and GTP on Ca2+ release.
- To assess if vitrification impacts these pathways.
Main Methods:
- Analysis of Ca2+ release from intracellular stores.
- Utilized inhibitory analysis with specific agents.
- Compared fresh and vitrified porcine oocytes.
Main Results:
- Somatotropin and GTP individually stimulated Ca2+ release.
- Combined somatotropin and GTP enhanced Ca2+ release in both fresh and vitrified oocytes.
- Inhibitors of protein kinase C and microtubules influenced Ca2+ depot interactions, but pathways remained consistent post-vitrification.
Conclusions:
- Signal transduction pathways in porcine oocytes are not altered by vitrification.
- The identified pathways involving somatotropin, GTP, and Ca2+ release are robust to cryopreservation.
