Related Experiment Videos
A novel flow cytometric method for measuring protein digestion within the phagocytic vacuole of polymorphonuclear
A P Haynes1, J Fletcher, M Garnett
1Department of Haematology, Medical Research Centre, City Hospital, Nottingham, U.K.
Journal of Immunological Methods
|December 31, 1990
Abstract:
When rhodamine is attached to albumin at a high molar ratio its fluorescence is quenched but fluorescence is released when the protein is digested and the dye released. Using this observation it is possible to measure protein digestion within the phagocytic vacuole of neutrophils. The assay is simple, rapid and measures digestion even in the presence of abnormal phagocytosis.