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[Comparative study on the content of chlorogenic acid in Solanum torvum from different origin, different harvest time
Yang He1, Hong-Bo Zhou, Jian-Xiong Liu
1Zhongshan Campus of Guangdong Pharmaceutical University, Zhongshan 528458, China. heyang626@163.com
Objective:
To develop a HPLC method for determination of chlorogenic acid in Solanum torvum and provide a scientific basis for evaluating the quality and reasonable utilization of the herb.
Methods:
HPLC was used to quantitatively determine the chlorogenic acid content in Solanum torvum from different origin, different harvest time and different part. The assay was performed on a Agela Promosil C18 (4.6 mm x 250 mm, 5 microm) column and eluted with a mobile phase consisted of acetonitrile -0.1% phosphoric acid solution at a flow rate of 1.0 ml/min. The column temperature was set at 35 degrees C. The detection wavelength was 327 nm.
Results:
The calibration curve was linear within the range of 0.1000 - 200.0 microg/mL (r = 0.9999). The average recoveries were 99.8% (RSD = 0.71%). The contents of chlorogenic acid in Solanum torvum were different from different origin, different harvest time and different part.
Conclusion:
The method is reliable, accurate and specific. It can be used for quality control of Solanum torvum and reasonable utilization of the herb.