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Updated: May 21, 2026

Validation of Therapeutic Agent Conjugation to Polyvinyl Alcohol-Coated Medical Devices
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Published on: November 29, 2024

Therapeutic proteins.

Dimiter S Dimitrov1

  • 1Protein Interactions Group, Frederick National Laboratory for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, MD, USA. dimiter.dimitrov@nih.gov

Methods in Molecular Biology (Clifton, N.J.)
|June 28, 2012
PubMed
Summary
This summary is machine-generated.

Protein therapeutics, including monoclonal antibodies (mAbs), are vital in medicine, with numerous approved drugs and many in development. Overcoming challenges in protein therapy requires new methodological advancements.

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Laboratory Scale Production and Purification of a Therapeutic Antibody
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Last Updated: May 21, 2026

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Published on: November 29, 2024

Laboratory Scale Production and Purification of a Therapeutic Antibody
09:54

Laboratory Scale Production and Purification of a Therapeutic Antibody

Published on: January 24, 2017

Area of Science:

  • Biotechnology
  • Pharmacology
  • Drug Development

Background:

  • Protein-based therapeutics are a cornerstone of modern medicine, with over 100 approved drugs and significant market sales.
  • Monoclonal antibodies (mAbs) represent a major class, accounting for nearly half of all therapeutic protein sales.
  • These therapies target diverse conditions including cancers, immune disorders, and infections.

Purpose of the Study:

  • To review the current landscape of protein-based therapeutics.
  • To categorize protein drugs by pharmacological activity, molecular type, and mechanism of action.
  • To identify the need for novel methodologies to address existing challenges in protein therapy.

Main Methods:

  • Classification of therapeutic proteins based on pharmacological activity (e.g., replacement, augmentation, novel function).
  • Categorization by molecular type (e.g., mAbs, Fc fusion proteins, enzymes, hormones).
  • Analysis of mechanisms of action (e.g., non-covalent binding, covalent interaction).

Main Results:

  • Protein therapeutics encompass a wide range of molecular types and mechanisms, with mAbs being a dominant class.
  • Hundreds of protein therapeutics are in clinical trials, with ongoing development of engineered proteins like bispecific mAbs.
  • Despite advancements, no fundamentally new methodological developments have emerged in recent decades.

Conclusions:

  • The field of protein therapeutics is mature and successful, but faces challenges like therapeutic resistance and target accessibility.
  • A paradigm shift in methodologies and a deeper understanding of biological mechanisms are crucial for future progress.
  • Continued innovation in protein engineering and delivery systems is essential to overcome current limitations.