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Related Concept Videos

DNA-only Transposons02:57

DNA-only Transposons

DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
What is Gene Expression?01:42

What is Gene Expression?

Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
What is Gene Expression?01:36

What is Gene Expression?

A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised  of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then processed and...
Microorganisms in Medicine and Therapeutics01:29

Microorganisms in Medicine and Therapeutics

Microorganisms play a fundamental role in vaccine development, gene therapy, and therapeutic production. Their biological properties are harnessed to advance medicine and public health. Beyond immunization, microorganisms contribute to gut health, antibiotic synthesis, and genetic disease treatment.Live Attenuated and Inactivated VaccinesLive attenuated vaccines, such as the measles, mumps, and rubella (MMR) vaccine, utilize weakened forms of pathogens to closely resemble natural infections.
Overview of Transposition and Recombination02:13

Overview of Transposition and Recombination

Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...
Transposons01:24

Transposons

Transposons, or "jumping genes," are small mobile genetic elements (MGEs) that range from 700 to 40,000 base pairs in length. They are found in all organisms and can move within the same chromosome or transfer to different chromosomes. In some cases, transposons can also jump between different host DNA molecules, such as plasmids or viruses, contributing to genetic variability.Barbara McClintock first discovered these mobile genetic elements in the 1940s while studying maize genetics, and she...

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Controllable Ion Channel Expression through Inducible Transient Transfection
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Controllable Ion Channel Expression through Inducible Transient Transfection

Published on: February 17, 2017

Transient expression technologies: past, present, and future.

Sabine Geisse1, Bernd Voedisch

  • 1Novartis Institutes for BioMedical Research, Basel, Switzerland. Sabine.Geisse@novartis.com

Methods in Molecular Biology (Clifton, N.J.)
|June 28, 2012
PubMed
Summary
This summary is machine-generated.

Transient gene expression in mammalian cells offers a rapid alternative to stable cell lines for producing recombinant proteins. This technology has evolved over the last decade, becoming more accessible for various research needs.

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Cell Biology

Background:

  • Mammalian cell systems were historically complex for protein production.
  • Stable cell line development is time-consuming and labor-intensive.
  • Transient gene expression emerged as a faster alternative.

Purpose of the Study:

  • To review the development of transient gene expression technologies over the past decade.
  • To summarize the current status of these expression protocols.
  • To provide an outlook on future trends in transient expression.

Main Methods:

  • Review of historical and recent literature on transient gene expression protocols.
  • Analysis of technological advancements and optimizations.
  • Discussion of current applications and future potential.

Main Results:

  • Transient gene expression protocols have significantly improved over the last 10 years.
  • These methods are now more accessible and adaptable to different laboratory settings.
  • Transient expression provides a viable alternative for rapid recombinant protein generation.

Conclusions:

  • Transient gene expression has become a crucial tool in biotechnology.
  • Continued optimization will further enhance its utility in research and industry.
  • Future developments may focus on increased efficiency and broader applicability.