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[The multiple effect of plasmid RP4::Mu cts 62 in transcipient bacilli].
Mikrobiologicheskii Zhurnal
|July 1, 1990
Summary
Conjugative plasmid transfer from Escherichia coli to Bacillus species negatively impacts cell vitality, growth, and structure. This genetic exchange leads to accelerated cell death and membrane disruption in various Bacillus strains.
Area of Science:
- Microbiology
- Molecular Biology
- Genetics
Background:
- Conjugative plasmids facilitate horizontal gene transfer between bacteria.
- Escherichia coli is a well-studied model organism for bacterial genetics.
- Bacillus species are important in various industrial and environmental processes.
Purpose of the Study:
- To investigate the effects of conjugative plasmid RP4::Mu cts 62 transfer on Bacillus cells.
- To assess the impact on Bacillus cell physiology and structure.
Main Methods:
- Conjugative plasmid transfer from Escherichia coli to Bacillus cereus, Bacillus thuringiensis, Bacillus mesentericus, and Bacillus polymyxa.
- Observation of cellular changes in vitality, growth, reproduction, and ultrastructure.
Main Results:
- Plasmid transfer decreased Bacillus cell vitality.
- Autolytic cell decay was accelerated post-transfer.
- Cell growth and reproduction rates were delayed in both liquid and solid media.
- Disruption of the cell membrane and surface layer ultrastructure was observed.
Conclusions:
- The conjugative hybrid plasmid RP4::Mu cts 62 significantly alters Bacillus cell structure and physiology.
- Plasmid acquisition by Bacillus species can lead to reduced viability and impaired cellular functions.