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Updated: May 21, 2026

Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach
Published on: December 4, 2021
Effect of ion adsorption on CEC separation of small molecules using hypercrosslinked porous polymer monolithic
Xiao-Jia Chen1, Ngoc Phuoc Dinh, Jing Zhao
1State Key Laboratory of Quality Research in Chinese Medicine, University of Macau, Macao SAR, P R China.
Abstract:
Both poly(styrene-co-vinylbenzyl chloride-co-divinylbenzene) and poly(4-methylstyrene-co-vinylbenzyl chloride-co-divinylbenzene) monolithic columns have been hypercrosslinked and for the first time used to achieve capillary electrochromatographic separations. Although these columns do not contain ionizable functionalities, electroosmotic flow was observed due to adsorption of ions from a buffer solution contained in the mobile phase on the surface of the hydrophobic polymer. An increase of more than one order of magnitude was observed with the use of both monolithic polymers. The hypercrosslinking reaction creates a large surface area thus enabling adsorption of a much larger number of ions. Alkylbenzenes were successfully separated using the hypercrosslinked monolithic columns.
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