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Related Concept Videos

Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

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Related Experiment Video

Updated: May 21, 2026

Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus
09:57

Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus

Published on: December 28, 2016

RNA interference-mediated gene knockdown within specific cell types.

Changqing Zhang1, David W Galbraith

  • 1School of Plant Sciences, The University of Arizona, Tucson, AZ 85721, USA. cqzhang@mail.utexas.edu

Plant Molecular Biology
|June 29, 2012
PubMed
Summary
This summary is machine-generated.

RNA interference (RNAi) gene silencing in plants is often cell autonomous. This study shows RNAi-induced silencing does not spread to adjacent cells, enabling selective gene knockdown in specific plant cell types.

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Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

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Area of Science:

  • Plant molecular biology
  • Gene regulation
  • Cell-to-cell communication

Background:

  • RNA interference (RNAi) is a gene silencing mechanism in plants.
  • Gene silencing signals can move cell-to-cell and over long distances in plants.
  • Understanding the spread of RNAi is crucial for its application in gene regulation.

Purpose of the Study:

  • To investigate the cell-autonomous nature of RNAi-induced gene silencing in Arabidopsis root tips.
  • To determine if dsRNA-induced silencing spreads to adjacent cells.
  • To assess the feasibility of cell-type-specific gene knockdown using RNAi.

Main Methods:

  • Utilized nuclear-localized GFP fusion protein to visualize gene silencing patterns.
  • Generated transgenic Arabidopsis expressing dsRNA targeting specific genes or marker genes.
  • Analyzed gene expression in root tip cells, including endodermis, CEI, and QC cells.

Main Results:

  • dsRNA-induced silencing was restricted to the cells expressing the dsRNA transgenes.
  • Silencing did not spread from the initiation site to adjacent cells in any of the experimental setups.
  • Selective gene knockdown was achieved specifically within QC and endodermal cells.

Conclusions:

  • RNAi-induced gene silencing in Arabidopsis root tips is a cell-autonomous process.
  • The spread of silencing signals is limited, preventing systemic effects.
  • This study demonstrates the potential for precise, cell-type-specific gene knockdown using RNAi in plants.