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The effect of oxiferriscorbone on striatal adenylate cyclase of the rat
1Department of Neurology, Medical University of Lübeck, F.R.G.
Abstract:
The effects of oxiferriscorbone on basal and forskolin-stimulated activity of adenylate cyclase in membrane preparations from caudate-putamen of the rat have been studied. Oxiferriscorbone, at 30 microM, stimulated the basal activity of the enzyme, but dose-dependently inhibited forskolin-activated activity of adenylate cyclase. Pertussis toxin was found to antagonize this inhibitory effect of oxiferriscorbone. Dopamine stimulated the activity of adenylate cyclase in the striatum, as described previously. When assayed together, the stimulating effects of dopamine and oxiferriscorbone were were additive, implying that they do not act at identical sites. The D1 receptor antagonist, alpha (+)-flupentixol completely blocked the effect of dopamine but had no significant effect on oxiferriscorbone-induced stimulation. The present results suggest that interaction between oxiferriscorbone and the inhibitory guanine nucleotide subunit. However, further studies are necessary.