Related Experiment Video
Updated: May 21, 2026
10:51
Isolation and Culture of Primary Mouse Keratinocytes from Neonatal and Adult Mouse Skin
Published on: July 14, 2017
Ultraviolet B-induced LGI3 secretion protects human keratinocytes
Seung Hoon Lee1, Yun-Mi Jeong, So-Young Kim
1Department of Biochemistry, Chung-Ang University College of Medicine, Dongjak-gu, Seoul, Republic of Korea.
Experimental Dermatology
|June 30, 2012
Summary
Leucine-rich glioma inactivated 3 (LGI3) is newly found in human keratinocytes. This protein enhances skin cell survival after UVB exposure by regulating Akt and p53 pathways, suggesting a role in skin homeostasis.
Area of Science:
- Dermatology
- Molecular Biology
- Cell Biology
Background:
- Leucine-rich glioma inactivated 3 (LGI3) is primarily known for its expression in the brain.
- The role and expression of LGI3 in skin cells, particularly keratinocytes, were previously uncharacterized.
Discussion:
- LGI3 is expressed in normal human keratinocytes and its secretion increases upon UVB exposure.
- LGI3 promotes keratinocyte survival post-UVB irradiation by activating the Akt signaling pathway.
- LGI3 influences p53 levels through MDM2 phosphorylation and subsequent degradation, indicating a regulatory role in the p53 pathway.
Key Insights:
- First-time identification of LGI3 expression in human keratinocytes.
- Demonstration of LGI3's protective effect on keratinocytes against UVB-induced damage.
- Elucidation of LGI3's mechanism involving Akt and p53 signaling pathways.
Outlook:
- LGI3 emerges as a novel cytokine involved in maintaining skin homeostasis.
- Further research could explore LGI3's therapeutic potential in skin conditions related to UV damage or impaired cell survival.
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