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Imaging Biological Samples with Optical Microscopy

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Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
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Related Experiment Video

Updated: May 20, 2026

Substructure Analyzer: A User-Friendly Workflow for Rapid Exploration and Accurate Analysis of Cellular Bodies in Fluorescence Microscopy Images

Substructure Analyzer: A User-Friendly Workflow for Rapid Exploration and Accurate Analysis of Cellular Bodies in Fluorescence Microscopy Images

Published on: July 15, 2020

A call for bioimaging software usability.

Anne E Carpenter¹, Lee Kamentsky, Kevin W Eliceiri

¹Imaging Platform, Broad Institute of Massachusetts Institute of Technology and Harvard, Cambridge, Massachusetts, USA. anne@broadinstitute.org

|June 30, 2012

Summary

This summary is machine-generated.

Bioimaging software often remains unused due to poor usability. Prioritizing user-friendly design in bioimaging software development is crucial for maximizing research and public investment.

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Area of Science:

Life Sciences
Biotechnology
Scientific Computing

Background:

Bioimaging software developed in research settings frequently faces limited adoption within the broader scientific community.
This lack of widespread use can hinder scientific progress and represents an inefficient use of resources.

Purpose of the Study:

To advocate for increased emphasis on usability in bioimaging software development.
To identify and describe key characteristics of usability that bioimaging software projects should target.

Main Methods:

Qualitative analysis of factors contributing to software adoption.
Literature review on human-computer interaction principles in scientific software.
Expert consultation on best practices for bioimaging software design.

Main Results:

Usability is a critical, yet often undervalued, factor in the adoption of bioimaging tools.
Specific, actionable usability characteristics can guide developers toward creating more impactful software.
Addressing usability issues can enhance the return on investment for both researchers and the public.

Conclusions:

Elevating usability as a primary development goal is essential for bioimaging software.
Adopting defined usability characteristics will lead to wider software dissemination and impact.
Improved usability ensures that scientific software investments yield greater returns.