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Updated: May 20, 2026

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Long-term Intravital Immunofluorescence Imaging of Tissue Matrix Components with Epifluorescence and Two-photon Microscopy
Published on: April 22, 2014
Three-dimensional second-harmonic generation imaging of fibrillar collagen in biological tissues
Jiansong Xie1, John Ferbas, Gloria Juan
1Department of Medical Sciences, Amgen, Thousand Oaks, California, USA.
Current Protocols in Cytometry
|July 4, 2012
Abstract:
Multiphoton-induced second-harmonic generation (SHG) has developed into a very powerful approach for in depth visualization of some biological structures with high specificity. In this unit, we describe the basic principles of three-dimensional SHG microscopy. In addition, we illustrate how SHG imaging can be utilized to assess collagen fibrils in biological tissues. Some technical considerations are also addressed.

