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Related Experiment Video

Updated: May 20, 2026

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy (ATOM)
07:19

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy (ATOM)

Published on: June 28, 2017

High-throughput single-microparticle imaging flow analyzer.

Keisuke Goda1, Ali Ayazi, Daniel R Gossett

  • 1Department of Electrical Engineering, University of California, Los Angeles, CA 90095, USA. goda@ee.ucla.edu

Proceedings of the National Academy of Sciences of the United States of America
|July 4, 2012
PubMed
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We developed a high-throughput optical microscope for rapid, precise screening of millions of microparticles. This automated system achieves 100,000 particles/s, enabling efficient analysis of biological samples like yeast and cancer cells.

Area of Science:

  • Biomedical Engineering
  • Optical Physics
  • Microfluidics

Background:

  • Conventional optical microscopy has limitations in high-throughput screening due to low speed and memory constraints.
  • Analyzing large populations of microscopic entities requires advanced imaging techniques beyond traditional methods.

Purpose of the Study:

  • To develop an automated, flow-through single-particle optical microscope for high-throughput screening.
  • To overcome the throughput limitations of conventional microscopy for large-scale particle analysis.

Main Methods:

  • Integration of ultrafast optical imaging, self-focusing microfluidics, and optoelectronic communication.
  • Development of a system for blur-free image acquisition and real-time classification of microparticles in high-speed flow.

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Simultaneous Measurement of Turbulence and Particle Kinematics Using Flow Imaging Techniques
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Simultaneous Measurement of Turbulence and Particle Kinematics Using Flow Imaging Techniques

Published on: March 12, 2019

Micro-particle Image Velocimetry for Velocity Profile Measurements of Micro Blood Flows
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Micro-particle Image Velocimetry for Velocity Profile Measurements of Micro Blood Flows

Published on: April 25, 2013

Related Experiment Videos

Last Updated: May 20, 2026

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy (ATOM)
07:19

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy (ATOM)

Published on: June 28, 2017

Simultaneous Measurement of Turbulence and Particle Kinematics Using Flow Imaging Techniques
10:53

Simultaneous Measurement of Turbulence and Particle Kinematics Using Flow Imaging Techniques

Published on: March 12, 2019

Micro-particle Image Velocimetry for Velocity Profile Measurements of Micro Blood Flows
07:53

Micro-particle Image Velocimetry for Velocity Profile Measurements of Micro Blood Flows

Published on: April 25, 2013

Main Results:

  • Achieved an unprecedented throughput of 100,000 particles/s.
  • Demonstrated high-throughput screening of budding yeast and rare breast cancer cells.
  • Attained a record low false positive rate of one in a million.

Conclusions:

  • The automated flow-through microscope significantly enhances screening capabilities for microscopic particles.
  • This technology enables precise, large-scale analysis of biological samples, including rare cell detection.