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Related Concept Videos

Hybridoma Technology01:31

Hybridoma Technology

Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation, polyethylene glycol...

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Related Experiment Video

Updated: May 20, 2026

Microfluidic Approach to Resolve Simultaneous and Sequential Cytokine Secretion of Individual Polyfunctional Cells
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Published on: March 8, 2024

Functional single-cell hybridoma screening using droplet-based microfluidics.

Bachir El Debs1, Ramesh Utharala, Irina V Balyasnikova

  • 1Institut de Science et d'Ingénierie Supramoléculaires, Université de Strasbourg, 8 allée Gaspard Monge, 67083 Strasbourg Cedex, France.

Proceedings of the National Academy of Sciences of the United States of America
|July 4, 2012
PubMed
Summary
This summary is machine-generated.

This study introduces a microfluidic platform for rapid functional screening of up to 300,000 hybridoma clones per day, accelerating the discovery of therapeutic monoclonal antibodies.

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Area of Science:

  • Biotechnology
  • Immunology
  • Drug Discovery

Background:

  • Monoclonal antibodies are a rapidly growing class of therapeutics.
  • Screening for antibody function is challenging and time-consuming.
  • Current methods limit screening to a few thousand clones over several weeks.

Purpose of the Study:

  • To develop a high-throughput microfluidic platform for functional screening of antibody-producing cells.
  • To enable direct functional screening without cell proliferation.
  • To accelerate the discovery of therapeutic monoclonal antibodies.

Main Methods:

  • Encapsulating individual cells into aqueous microdroplets.
  • Assaying antibody function via fluorescence detection within droplets.
  • Utilizing fluorescence-activated droplet sorting for enrichment.

Main Results:

  • Screened up to 300,000 hybridoma clones in under a day.
  • Achieved 9,400-fold enrichment of antibody-producing cells using fluorescence-activated droplet sorting.
  • Demonstrated applicability to non-immortalized primary B-cells.

Conclusions:

  • The microfluidic platform significantly enhances the efficiency of functional antibody screening.
  • This technology can accelerate the development of novel antibody-based therapeutics.
  • The system allows for direct screening of primary B-cells, expanding therapeutic discovery potential.