Related Experiment Videos
Structure of the amino acid-containing capsular polysaccharide from Escherichia coli O8:K49:H21
L M Beynon1, G G Dutton, J C Richards
1Department of Chemistry, University of British Columbia, Vancouver, Canada.
Carbohydrate Research
|September 19, 1990
Abstract:
The structure of the capsular antigen of E. coli K49 and the oligosaccharides derived from it by partial acid hydrolysis were studied by 1D- and 2D-n.m.r. spectroscopy, g.l.c.-c.i.-mass spectrometry, and methylation analysis. The K49 polysaccharide consists of the repeating unit----4)-beta-D-GlcpA-(1----6)-beta-D-Galp-(1----6)-beta-D-Glcp- (1----3)-beta - D-GalpNAc-(1----. The glucuronic acid residues are substituted, in the apparent molar ratio of 4:1, with L-threonine and L-serine linked amidically to the carboxyl group.