Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Clinical Applications of Epidermal Stem Cells

Clinical Applications of Epidermal Stem Cells

Epidermal stem cells (EpiSCs) are mainly located at the basal layer of the epidermis. These cells repair minor injuries of the skin and replace dead skin cells. However, EpiSCs’ cannot heal severe wounds such as major burns or those from diabetes or hereditary disorders. In such cases, culturing the epidermal stem cells from the patient is possible and has yielded successful treatment options, such as laboratory-grown skin grafts. These grafts are synthesized using a patient’s own EpiSCs...

Renewal of Skin Epidermal Stem Cells

Renewal of Skin Epidermal Stem Cells

The skin is divided into epidermis, dermis, and hypodermis, the skin's outermost, middle, and inner layers. The human epidermal layer regularly undergoes renewal, where old, dead cells are replaced by new cells. Epidermal stem cells or EpiSCs divide and differentiate to restore the lost cells. For the renewal process, some EpiSCs continuously self-renew. In contrast, few others differentiate into transit-amplifying cells, which later form prickle or spinous cells, followed by granular cells,...

Renewal of Intestinal Stem Cells

Renewal of Intestinal Stem Cells

The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the goblet,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Sequential fulminant hepatitis and toxic epidermal necrolysis induced by tislelizumab: A case report.

Experimental and therapeutic medicine·2026

Same author

B7-H3-mediated cis-inhibition of EGFR by a tumor-selective bispecific antibody enhances anti-tumor efficacy and minimizes toxicities.

Nature communications·2026

Same author

Cytoplasmic calcium influx mediated by Lr14a regulates stomatal immunity against leaf rust in wheat.

Current biology : CB·2025

Same author

Geographical analysis of malignant tumor incidence and treatment in China.

Scientific reports·2025

Same author

Live Cell Imaging for Keratinocyte Lineage Tracing.

Methods in molecular biology (Clifton, N.J.)·2025

Same author

Generalized Boosted Models to Measure Racial Effects at Different Quantiles in Observational Studies.

Biometrical journal. Biometrische Zeitschrift·2025

Search research articles

Related Experiment Video

Updated: May 20, 2026

Patient Derived Cell Culture and Isolation of CD133+ Putative Cancer Stem Cells from Melanoma

Patient Derived Cell Culture and Isolation of CD133⁺ Putative Cancer Stem Cells from Melanoma

Published on: March 13, 2013

CD133 is a marker for long-term repopulating murine epidermal stem cells.

Alexandra Charruyer¹, Lauren R Strachan, Lili Yue

¹Department of Dermatology, University of California, San Francisco, San Francisco, California, USA.

The Journal of Investigative Dermatology

|July 6, 2012

Summary

Researchers identified specific mouse keratinocyte populations (CD133(+) and CD133(+)ΔΨm(hi)) as epidermal stem cells (EpiSCs). These EpiSCs demonstrate self-renewal, multipotency, and long-term epidermal regeneration capabilities.

More Related Videos

Isolation of CD133+ Liver Stem Cells for Clonal Expansion

Isolation of CD133+ Liver Stem Cells for Clonal Expansion

Published on: October 10, 2011

Mouse Epidermal Neural Crest Stem Cell (EPI-NCSC) Cultures

Mouse Epidermal Neural Crest Stem Cell (EPI-NCSC) Cultures

Published on: May 9, 2008

Related Experiment Videos

Last Updated: May 20, 2026

Patient Derived Cell Culture and Isolation of CD133+ Putative Cancer Stem Cells from Melanoma

Patient Derived Cell Culture and Isolation of CD133⁺ Putative Cancer Stem Cells from Melanoma

Published on: March 13, 2013

Isolation of CD133+ Liver Stem Cells for Clonal Expansion

Isolation of CD133+ Liver Stem Cells for Clonal Expansion

Published on: October 10, 2011

Mouse Epidermal Neural Crest Stem Cell (EPI-NCSC) Cultures

Mouse Epidermal Neural Crest Stem Cell (EPI-NCSC) Cultures

Published on: May 9, 2008

Area of Science:

Dermatology
Stem Cell Biology
Cell Biology

Background:

Epidermal homeostasis relies on epidermal stem cells (EpiSCs).
Isolating pure stem cell populations is crucial for understanding tissue regeneration.
Previous research has not definitively identified and isolated functional EpiSCs.

Purpose of the Study:

To identify and isolate functional epidermal stem cells (EpiSCs) from murine keratinocytes.
To assess the self-renewal, multipotency, and long-term repopulating capacity of isolated cell populations.
To characterize EpiSCs based on specific cell surface markers and functional assays.

Main Methods:

Utilized fluorescence-activated cell sorting (FACS) to isolate putative EpiSC populations.
Employed quantitative transplantation assays with limiting dilution analysis for repopulating ability assessment.
Conducted serial transplantation to evaluate self-renewal capacity and assessed multipotency through hair follicle production.

Main Results:

CD133(+) and CD133(+)ΔΨm(hi) keratinocyte populations showed significantly enriched long-term epidermal repopulating activity (3.9- and 5.2-fold, respectively).
Serial transplantation confirmed self-renewal capacity in CD133(+) and CD133(+)ΔΨm(hi) derived epidermal repopulating units.
CD133(+) keratinocytes exhibited multipotency, producing more hair follicles than CD133(-) cells, and contained label-retaining cells.

Conclusions:

Murine keratinocytes within CD133(+) and CD133(+)ΔΨm(hi) populations contain functional epidermal stem cells (EpiSCs).
These identified EpiSCs possess long-term regenerative potential, self-renewal, multipotency, and label-retaining characteristics.
This study provides a method for isolating and characterizing EpiSCs, advancing cutaneous biology research.