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Sizing of the Leptospira genome by pulsed-field agarose gel electrophoresis
1Unité des Leptospires, Institut Pasteur, Paris, France.
FEMS Microbiology Letters
|September 1, 1990
Abstract:
Pulsed-field gel electrophoresis allowed the determination of the size of the genome of Leptospira, a bacterium of the spirochete family. The three restriction enzymes, NotI (5'GC/GGCCGC), NheI (5'G/CTAGC), ApaI (5'-GGGCC/C) generated DNA fragments of suitable size. The results are compatible with a size of 5000 kb for the chromosome of both the pathogenic and the saprophytic species of Leptospira.