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Updated: May 20, 2026

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
Published on: April 23, 2019
Benzodiazepines and metabolites from biological fluids by liquid chromatography electrospray tandem mass spectrometry
Cynthia L Morris-Kukoski1, Jason E Schaff, Louis J Reda
1Chemistry Unit, FBI Laboratory, Quantico, VA, USA. Cynthia.Morris-Kukoski@ic.fbi.gov
Abstract:
Solid-phase extraction and liquid chromatography-tandem mass spectrometry are invaluable techniques for the determination of benzodiazepines and metabolites in biological matrices. The reason for using tandem mass spectrometry is to increase limits of detection without the need for chemical derivatization. Here we describe a technique for the detection of 26 benzodiazepines and metabolites at a detection limit of approximately 1-2 ng/mL in blood and 1-5 ng/mL in urine when screened using a data-dependent scan method.
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