Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
Complexometric Titration: Ligands00:43

Complexometric Titration: Ligands

Different monodentate and polydentate ligands are used as complexing agents in complexometric titration reactions. The formation of complexes by mono- and bidentate ligands involves two or more intermediate steps, limiting their use as complexing agents. In comparison, polydentate ligands can form complexes with metal ions in a single-step process, facilitating sharper end points. This means polydentate ligands, such as amino carboxylic acid derivatives, are most commonly employed in...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Impact of Three Single-Step Decontamination Methods on Respirators and Medical Gowns.

Biomedical instrumentation & technology·2026
Same author

Single-stage laparoscopic management of gallstone ileus with concurrent Bouveret syndrome: a case report.

International journal of surgery case reports·2026
Same author

Negative Pressure Wound Therapy for the Prevention of Wound Complications After Hepatopancreatobiliary Surgery: A Systematic Review and Meta-Analysis.

Health science reports·2026
Same author

Liposome-cyclodextrin synergistic system for enhanced chiral separation of Fmoc-amino acid enantiomers in capillary electrophoresis.

Mikrochimica acta·2026
Same author

Small auxin-up RNA promotes <i>GmMYB176</i> gene transcription to modulate seed isoflavone accumulation in soybean.

Frontiers in plant science·2026
Same author

Multi-locus genome-wide association study on the rheological traits in wheat flour dough.

BMC genomics·2026

Related Experiment Video

Updated: May 20, 2026

Study of Short Peptide Adsorption on Solution Dispersed Inorganic Nanoparticles Using Depletion Method
09:43

Study of Short Peptide Adsorption on Solution Dispersed Inorganic Nanoparticles Using Depletion Method

Published on: April 11, 2020

Quantifying ligand adsorption to nanoparticles using tandem differential mobility mass analysis.

Suvajyoti Guha, Xiaofei Ma, Michael J Tarlov

    Analytical Chemistry
    |July 10, 2012
    PubMed
    Summary

    A new method combines electrospray-differential mobility analyzer (ES-DMA) with a particle mass analyzer (APM) for accurate nanoparticle ligand quantification. This technique overcomes limitations of previous methods, enabling precise mass analysis regardless of particle properties.

    More Related Videos

    Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance (SPR)
    09:35

    Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance (SPR)

    Published on: November 29, 2014

    Mass-Sensitive Particle Tracking to Characterize Membrane-Associated Macromolecule Dynamics
    13:30

    Mass-Sensitive Particle Tracking to Characterize Membrane-Associated Macromolecule Dynamics

    Published on: February 18, 2022

    Related Experiment Videos

    Last Updated: May 20, 2026

    Study of Short Peptide Adsorption on Solution Dispersed Inorganic Nanoparticles Using Depletion Method
    09:43

    Study of Short Peptide Adsorption on Solution Dispersed Inorganic Nanoparticles Using Depletion Method

    Published on: April 11, 2020

    Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance (SPR)
    09:35

    Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance (SPR)

    Published on: November 29, 2014

    Mass-Sensitive Particle Tracking to Characterize Membrane-Associated Macromolecule Dynamics
    13:30

    Mass-Sensitive Particle Tracking to Characterize Membrane-Associated Macromolecule Dynamics

    Published on: February 18, 2022

    Area of Science:

    • Nanotechnology and Materials Science
    • Analytical Chemistry
    • Biophysics

    Background:

    • Electrospray-differential mobility analyzers (ES-DMA) are used for nanoparticle characterization but struggle with accurate ligand quantification due to conformational effects.
    • Previous methods for analyzing ligand binding to nanoparticles often yield inaccurate surface coverage measurements.
    • Direct mass analysis independent of particle size, material, morphology, and conformation is needed for reliable quantification.

    Discussion:

    • The study introduces an in-flight coupling of a particle mass analyzer (APM) with ES-DMA for direct quantitative analysis.
    • This novel ES-DMA-APM technique enables mass determination independent of particle characteristics, including conformation.
    • The method was validated using model systems: gold nanoparticles with bovine serum albumin and polystyrene beads with antibodies.

    Key Insights:

    • The ES-DMA-APM technique provides accurate, mass-based quantification of ligand binding to nanoparticles.
    • Conformational effects, a limitation in prior ES-DMA analyses, are overcome by this new method.
    • Results from model systems show excellent agreement with existing data, confirming the technique's reliability.

    Outlook:

    • The developed ES-DMA-APM method significantly enhances online quantitative characterization capabilities for nanoparticle-ligand interactions.
    • This advancement is expected to improve the understanding and application of functionalized nanoparticles in various fields.
    • Future research can leverage this technique for more precise analysis of complex biological and synthetic nanoparticle systems.