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Updated: May 20, 2026

An Automated Culture System for Maintaining and Differentiating Human-Induced Pluripotent Stem Cells
Published on: January 26, 2024
Epigenetic alterations in human pluripotent stem cells: a tale of two cultures
1Wellcome Trust Centre for Stem Cell Research, Stem Cell Institute, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK. aw512@cam.ac.uk
Abstract:
Human somatic cells can be reprogrammed into induced pluripotent stem cells (hiPSCs) with wide lineage differentiation potential in culture. However, reprogramming and long-term culture can also induce abnormalities in these pluripotent cells. This minireview discusses recent studies that have identified changes in imprinted gene expression and erosion of X chromosome inactivation in female hiPSCs and how understanding the sources and consequences of epigenetic variability in hiPSCs will impact disease modeling and clinical application in the future.
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