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Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...

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Updated: May 20, 2026

Single-Molecule Imaging of Nuclear Transport

Single-Molecule Imaging of Nuclear Transport

Published on: June 9, 2010

Nuclear pore dynamics during the cell cycle.

Naoko Imamoto¹, Tomoko Funakoshi

¹Cellular Dynamics Laboratory, Riken Advanced Science Institute, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan. nimamoto@riken.jp

Current Opinion in Cell Biology

|July 10, 2012

Summary

Nuclear pore complexes (NPCs) regulate molecule transport between the nucleus and cytoplasm. Their assembly, disassembly, and distribution change dynamically during the cell cycle, with NPCs becoming highly stable after cell division.

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Last Updated: May 20, 2026

Single-Molecule Imaging of Nuclear Transport

Single-Molecule Imaging of Nuclear Transport

Published on: June 9, 2010

Area of Science:

Cell Biology
Molecular Biology
Biochemistry

Background:

Nuclear pore complexes (NPCs) are essential large protein assemblies facilitating nucleocytoplasmic transport.
NPCs undergo dynamic changes in assembly, disassembly, and distribution throughout the cell cycle.
NPC stability significantly increases in the post-mitotic phase.

Purpose of the Study:

To investigate the molecular mechanisms governing NPC assembly and disassembly.
To understand how NPC dynamics are regulated during the cell cycle.
To explore the relationship between NPC localization and nuclear envelope structure.

Main Methods:

Molecular level studies of NPC components and building blocks.
Analysis of NPC assembly and disassembly mechanisms.
Investigation of cell cycle-dependent NPC dynamics and regulation.

Main Results:

NPC assembly and disassembly mechanisms vary based on the initial localization of components (e.g., chromatin vs. nuclear envelope).
NPC dynamics are intricately linked with inner nuclear membrane proteins, lamins, lipid membranes, and cell cycle signaling.
NPCs exhibit distinct regulatory processes influenced by cell cycle progression.

Conclusions:

NPC assembly and disassembly are complex, regulated processes influenced by component origin and cell cycle stage.
The dynamic behavior of NPCs is tightly controlled and integrated with other nuclear envelope components and signaling pathways.
Understanding NPC regulation is crucial for comprehending nuclear envelope function and cell division.