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Thin-layer Chromatographic (TLC) Separations and Bioassays of Plant Extracts to Identify Antimicrobial Compounds
Published on: March 27, 2014
Thin layer chromatography in the separation of unsaturated organic compounds using silver-thiolate chromatographic
James T Dillon1, José C Aponte2, Yih-Jin Tsai2
1Department of Chemistry, Brown University, 324 Brook Street, Providence, RI 02906, USA.
Abstract:
We report the use of silver-thiolate chromatographic material (AgTCM) as a stable material for use in TLC. The AgTCM stationary phase operates under the same principles as silver-ion chromatography, separating compounds by degree of unsaturation; however, the AgTCM stationary phase shows considerable advantages over Ag-TLC in terms of light stability and shelf lifetime. We demonstrate the light stability of the AgTCM-TLC and its application for separations based on the degrees of unsaturation using fatty acid methyl esters (FAMEs) and polycyclic aromatic hydrocarbons (PAHs).
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