Trifolium species-derived substances and extracts--biological activity and prospects for medicinal applications

Despite of the fact that clovers (family: Fabaceae; genus: Trifolium) have been known for many centuries as important forage plants and valuable herbs in folk medicine, their phytochemical characteristics and biological activity remain only partly established.

The presentation of the current knowledge of physiological effects, therapeutic action, new trends in the investigation of Trifolium plants and suggestions for the future applications of these herbs in therapy of various disorders.

A critical review of literature on the biological activity of Trifolium plants, with the indication on important gaps, was performed. The compilation of existing information on physiological effects and medicinal value of clovers, derived from both traditional medicine recommendations and scientific reports, is presented.

The available data indicate on the abundance of biologically active substances in Trifolium plants, including numerous flavonoids, saponins, clovamides and phenolic acids. The best known clover - Trifolium pratense L. (red clover) - is used for the production of herbal medicines, an alternative to the conventional hormonal replacement therapy. The biological activity and potential therapeutic effects of other Trifolium species have gained a considerable scientific interest; extracts obtained from various clovers have been shown to possess antioxidative and antiinflammatory activities, inhibiting angiogenesis and displaying anti-cancer properties.

Clovers other than T. pratense also seem to be a promising source of valuable phytochemicals, such as isoflavones and various flavonoids. However, the therapeutic use of these Trifolium species is significantly limited by the lack of clinical evidence; thus further studies are needed.