Rethinking the role of TFIIF in transcript initiation by RNA polymerase II

TFIIF is considered to be a general transcription factor, based on the fact that it is essential for assembly of RNA polymerase II preinitiation complexes on fully double-stranded templates in vitro. Existing models assign various tasks to TFIIF during preinitiation complex formation and transcript initiation. Recent results do not support all aspects of those models but they do emphasize the significance of the interaction of TFIIF and TFIIB. Other recent findings raise the possibility that a fraction of RNA polymerase II transcription complex assembly proceeds through a pathway that is independent of TFIIF.