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Updated: May 20, 2026

Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy (FSM)
Published on: August 5, 2009
Distance measurements by speckle correlation of objective speckle patterns, structured by the illumination
Michael Linde Jakobsen1, Steen Grüner Hanson
1DTU Fotonik, Department of Photonics Engineering, Technical University of Denmark, P.O. Box 49, DK-4000, Roskilde, Denmark. mlja@fotonik.dtu.dk
Abstract:
Objective speckles produced by two beams overlapping and interfering on a rough object surface contain information about the angle of incidence of the two beams, and how well they overlap. We obtain the autocovariance function for such a speckle pattern, and demonstrate how the information carried by the objective speckles can be used to probe the distance between the object and the observation plane. From a distance of 75 mm to a distance of 150 mm, and using an angle of 0.3 deg between the two incident beams, we can measure the actual distance with an uncertainty of better than ±0.1% of the full range. As long as the beams overlap at the object surface, the proposed method can measure distance with an uncertainty inversely proportional to the spot size at the object.

