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Related Concept Videos

RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
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Ribosome Profiling

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Next-generation Sequencing

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The human body harbors a vast and diverse viral community known as the human virome. The virome includes bacteriophages that infect bacteria, and eukaryotic viruses that infect human cells. Transient dietary and environmental viruses also contribute to this dynamic ecosystem. Estimates suggest the human body may contain on the order of 10¹³ viral particles, though abundance varies widely by body site and detection method.Comprehensive characterization of the virome has become possible only with...

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Related Experiment Video

Updated: May 20, 2026

Collection and Extraction of Saliva DNA for Next Generation Sequencing
06:58

Collection and Extraction of Saliva DNA for Next Generation Sequencing

Published on: August 27, 2014

The human salivary RNA transcriptome revealed by massively parallel sequencing.

Nadine Spielmann1, Diane Ilsley, Jian Gu

  • 1Dental Research Institute, UCLA School of Dentistry, Los Angeles, CA, USA.

Clinical Chemistry
|July 10, 2012
PubMed
Summary

Saliva analysis using massively parallel sequencing reveals a complex RNA network, preserving structural integrity and identifying over 4000 genes and 400 microbial species. This salivary transcriptome offers a powerful new diagnostic tool.

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Last Updated: May 20, 2026

Collection and Extraction of Saliva DNA for Next Generation Sequencing
06:58

Collection and Extraction of Saliva DNA for Next Generation Sequencing

Published on: August 27, 2014

Single Read and Paired End mRNA-Seq Illumina Libraries from 10 Nanograms Total RNA
14:49

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Published on: October 27, 2011

Novel Sequence Discovery by Subtractive Genomics
09:40

Novel Sequence Discovery by Subtractive Genomics

Published on: January 25, 2019

Area of Science:

  • Molecular Biology
  • Genomics
  • Biotechnology

Background:

  • Salivary transcriptome analysis is an emerging diagnostic technology with disease detection capabilities.
  • Massively parallel sequencing provides nucleotide-level RNA information from saliva.

Purpose of the Study:

  • To explore massively parallel sequencing for comprehensive salivary transcriptome profiling.
  • To assess the diagnostic potential of salivary RNA for disease detection.

Main Methods:

  • RNA isolation from unstimulated cell-free saliva (CFS) and whole saliva (WS).
  • Transcriptome profiling using the SOLiD™ system.
  • Sequence alignment to the human genome and Human Oral Microbiome Database (HOMD).

Main Results:

  • Acquisition of complete nucleotide sequence information for the human salivary transcriptome.
  • Alignment of 20-25% of CFS reads to the human genome and 30% to HOMD.
  • Detection of >4000 coding and noncoding genes, with preserved transcript structural integrity.

Conclusions:

  • A single saliva sample yields comprehensive RNA sequence data for both human genes and microbial species.
  • Salivary RNA exhibits preserved structural integrity, contrary to previous findings.
  • The salivary transcriptome represents a complex RNA network with significant diagnostic potential.