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Fluorescence Anisotropy as a Tool to Study Protein-protein Interactions
Published on: October 21, 2016
Activity-based fluorescent probes for monitoring sulfatase activity
Hyun-Joo Park1, Hyun-Woo Rhee, Jong-In Hong
1Department of Chemistry, College of Natural Sciences, Seoul National University, Seoul, Republic of Korea.
Bioorganic & Medicinal Chemistry Letters
|July 11, 2012
Abstract:
A small-molecule probe for sulfatase is developed that shows a significant change in fluorescence upon reaction with sulfatase in an activity-based manner. As this probe is free from interference from background fluorescence caused by an unreacted probe, it could be a simple and efficient tool for the study of sulfatase activity.
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