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Updated: May 20, 2026

Aversive Associative Learning and Memory Formation by Pairing Two Chemicals in Caenorhabditis elegans
Published on: June 23, 2022
In vitro aversion conditioning in Lymnaea. Short communication
H Sunada1, K Lukowiak, M Sakakibara
1Graduate School of Bioscience, Tokai University, Numazu, Japan.
Abstract:
In an in vitro semi-intact Lymnaea preparation we were successful in using a training procedure to bring about Conditioning Taste Aversion (CTA ). Following paired presentation of the CS (sucrose) and US (tactile), the CS no longer elicits feeding. We can use sucrose to the lips in the semi-intact preparation as the CS; while we use direct current injection to depolarize RPeD11 as the US. Following pairing of these stimuli, the CS no longer elicits fictive feeding. We can determine the changes in synaptic input to neurons that play key roles in controlling feeding behavior.

