Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Neurotransmitters01:31

Neurotransmitters

Neurotransmitters are essential chemical messengers within the nervous system, facilitating the communication between neurons. These chemical messengers, varying in function and effect, are critical for sustaining various aspects of neurological health and emotional well-being.
Neurotransmitters01:30

Neurotransmitters

Neurotransmitters play a crucial role in the communication between neurons in the autonomic nervous system. Neurons in the autonomic nervous system can be cholinergic or adrenergic depending on the neurotransmitters synthesized. Cholinergic neurons use acetylcholine as their primary neurotransmitter. This includes all the preganglionic fibers of the sympathetic and pre- and postganglionic fibers of the parasympathetic nervous systems. In addition, neurons of the somatic nervous system also use...
Human Genetics01:28

Human Genetics

Human genetics provides a profound framework for understanding the interplay between genetic predispositions and human psychology. At the heart of this discipline lies the study of how genes influence physical traits, behaviors, and susceptibility to diseases. Each person carries a unique genetic code that subtly or significantly shapes their psychological and behavioral landscape.
The complex relationship between genetics and psychology is observable through common biological components such...
Neural Regulation01:37

Neural Regulation

Digestion begins with a cephalic phase that prepares the digestive system to receive food. When our brain processes visual or olfactory information about food, it triggers impulses in the cranial nerves innervating the salivary glands and stomach to prepare for food.
Drugs Affecting Neurotransmitter Synthesis01:29

Drugs Affecting Neurotransmitter Synthesis

Drugs affecting neurotransmitter synthesis can impact the adrenergic neuron and the synthesis of neurotransmitters. For example, α-methyltyrosine and carbidopa target specific enzymes involved in catecholamine synthesis. α-methyltyrosine inhibits the enzyme tyrosine hydroxylase, which converts tyrosine into dopamine. By blocking this enzyme, α-methyltyrosine reduces dopamine production and other catecholamines. Carbidopa, on the other hand, inhibits the enzyme dopa decarboxylase, which converts...
Drugs Affecting Neurotransmitter Release or Uptake01:21

Drugs Affecting Neurotransmitter Release or Uptake

Certain drugs can affect how neurotransmitters called catecholamines, are released or taken back up in the adrenergic neuron. They can have different effects on the body's sympathetic transmission. Reserpine, a natural compound found in the Rauwolfia shrub, blocks a transporter called vesicular monoamine transporter (VMAT), which leads to a buildup of catecholamines in the cell and reduces sympathetic transmission. Another drug called guanethidine works in multiple ways, including blocking...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Histamine Originating from the BNST Modulates Corticostriatal Synaptic Transmission during Early Postnatal Development.

The Journal of neuroscience : the official journal of the Society for Neuroscience·2026
Same author

Monitoring brain chemistry: electrochemical techniques and applications.

Bioelectrochemistry (Amsterdam, Netherlands)·2026
Same author

Monitoring Molecules in Neuroscience 2024.

ACS chemical neuroscience·2026
Same author

Nanomaterial Modification of Ultramicroelectrodes Using Design-of-Experiments Principles.

ACS electrochemistry·2026
Same author

Measurement of electrochemical brain activity with fast-scan cyclic voltammetry during functional magnetic resonance imaging.

Nature protocols·2025
Same author

Measurement of Real-Time Serotonin Dynamics from Human-Derived Gut Organoids.

Analytical chemistry·2025

Related Experiment Video

Updated: May 20, 2026

Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area
09:54

Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area

Published on: August 10, 2012

Brain dopamine and serotonin differ in regulation and its consequences.

Parastoo Hashemi1, Elyse C Dankoski, Rinchen Lama

  • 1Department of Chemistry, University of North Carolina, Chapel Hill, NC 27599, USA.

Proceedings of the National Academy of Sciences of the United States of America
|July 11, 2012
PubMed
Summary

Simultaneous in vivo voltammetry revealed serotonin (5-HT) release is more restricted than dopamine release. Serotonin transmission relies on uptake and degradation, unlike dopamine, and disrupting these can cause severe effects.

More Related Videos

Developing a Rat Model for Bipolar Disorder
04:42

Developing a Rat Model for Bipolar Disorder

Published on: May 2, 2025

A Plate-Based Assay for the Measurement of Endogenous Monoamine Release in Acute Brain Slices
07:56

A Plate-Based Assay for the Measurement of Endogenous Monoamine Release in Acute Brain Slices

Published on: August 11, 2021

Related Experiment Videos

Last Updated: May 20, 2026

Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area
09:54

Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area

Published on: August 10, 2012

Developing a Rat Model for Bipolar Disorder
04:42

Developing a Rat Model for Bipolar Disorder

Published on: May 2, 2025

A Plate-Based Assay for the Measurement of Endogenous Monoamine Release in Acute Brain Slices
07:56

A Plate-Based Assay for the Measurement of Endogenous Monoamine Release in Acute Brain Slices

Published on: August 11, 2021

Area of Science:

  • Neuroscience
  • Neurochemistry

Background:

  • Dopamine and serotonin (5-HT) are key neurotransmitters in psychological disorders.
  • In vivo detection of 5-HT using voltammetry is a recent advancement.

Purpose of the Study:

  • To simultaneously measure dopamine and 5-HT release in specific brain regions in vivo.
  • To compare the release mechanisms and regulation of dopamine and 5-HT.

Main Methods:

  • Simultaneous in vivo fast scan cyclic voltammetry using two carbon-fiber microelectrodes.
  • Measurement of dopamine in the nucleus accumbens and 5-HT in the substantia nigra pars reticulata in a single rat.
  • Physiological and pharmacological analyses.

Main Results:

  • 5-HT release was significantly more restricted than dopamine release, despite similar tissue levels.
  • 5-HT transmission is primarily regulated by uptake and metabolic degradation.
  • Dopamine transmission is mainly limited by synthesis and repackaging.
  • Inhibiting 5-HT uptake and degradation mimicked serotonin syndrome.

Conclusions:

  • Dopamine and serotonin exhibit distinct in vivo release dynamics and regulatory mechanisms.
  • Uptake and degradation are critical for controlling 5-HT levels.
  • Disruption of serotonergic regulation can lead to severe physiological consequences.