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Reversible light switch for macrocycle mobility in a DNA rotaxane.

Finn Lohmann1, Damian Ackermann, Michael Famulok

  • 1LIMES Institute, Chemical Biology & Medicinal Chemistry Unit, c/o Kekulé Institute of Organic Chemistry and Biochemistry, Gerhard-Domagk-Strasse 1, 53121 Bonn, Germany.

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Researchers developed dynamic DNA nanostructures that can reversibly switch between stationary and mobile states using strand displacement. This breakthrough enables controlled molecular motion in interlocked DNA architectures, paving the way for advanced nanotechnology applications.

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Area of Science:

  • DNA nanotechnology
  • Molecular engineering
  • Supramolecular chemistry

Background:

  • Dynamic DNA architectures are crucial for molecular motion.
  • Reversible processes are key for regenerating states in DNA nanostructures.
  • Interlocked DNA nanostructures offer unique mechanical properties.

Purpose of the Study:

  • To develop methods for reversible switching of DNA rotaxane architectures.
  • To enable controlled molecular motion in DNA nanostructures using external stimuli.
  • To explore the mechanical differences between stationary and mobile DNA states.

Main Methods:

  • Utilizing strand-displacement reactions for switching operations.
  • Employing toehold-extended oligodeoxynucleotides for one switching approach.
  • Using light-irradiation as an alternative switching mechanism.
  • Characterizing the reversible switching between two distinct topological states.

Main Results:

  • Two distinct approaches for reversible switching of a DNA rotaxane were successfully demonstrated.
  • Both stationary and mobile states were achieved with high fidelity through strand displacement.
  • Multiple cycles of back-and-forth switching between states were performed quantitatively.
  • The two states, though topologically similar, exhibited fundamentally different mechanical properties.

Conclusions:

  • Reversible mechanical motion can be achieved in interlocked DNA nanostructures.
  • This work opens new possibilities for designing dynamic and responsive DNA-based devices.
  • The developed methods offer precise control over molecular motion in DNA nanotechnology.