HPMC supplementation reduces abdominal fat content, intestinal permeability, inflammation, and insulin resistance in
Hyunsook Kim1, Glenn E Bartley, Scott A Young
1Department of Nutrition, University of California, Davis, CA, USA; USDA, ARS, Western Regional Research Center, Albany, CA 95616, USA. hyskim@ucdavis.edu
Scope:
The effects of hydroxypropyl methylcellulose (HPMC), a highly viscous nonfermentable soluble dietary fiber, were evaluated on adipose tissue inflammation and insulin resistance in diet-induced obese (DIO) mice fed a high-fat (HF) diet supplemented with either HPMC or insoluble fiber.
Methods And Results:
DIO C57BL/6J mice were fed a HF diet supplemented with 6% HPMC or 6% microcrystalline cellulose (MCC). Gene expression analyses of epididymal adipose tissue by exon microarray and real-time PCR along with glucose and insulin tolerance and intestinal permeability were assessed. HPMC-fed mice exhibited significantly reduced body weight gain and adipose tissue weight as well as reduced areas under the curve for 2-h insulin and glucose responses. HPMC significantly decreased HF diet-induced intestinal permeability. Overall, HPMC enhanced insulin sensitivity and glucose metabolism and downregulated genes related to inflammation and immune response, adipogenesis, and oxidative stress markers. Pathway analysis of microarray data identified lipid metabolism, inflammatory disease, and acute phase response pathways as being differentially regulated by HPMC.
Conclusion:
These results suggest HPMC consumption ameliorates HF diet effects on obesity-induced insulin resistance, adipose tissue inflammatory and immune responses, weight gain, as well as intestinal permeability.
Insights
Hydroxypropyl methylcellulose (HPMC) reduces diet-induced obesity, inflammation, and insulin resistance in mice. This soluble fiber improves glucose metabolism and gut barrier function, offering potential benefits for metabolic health.
Area of Science:
- Nutrition Science
- Metabolic Health
- Immunology
Background:
- Diet-induced obesity (DIO) is linked to adipose tissue inflammation and insulin resistance.
- High-fat (HF) diets exacerbate these metabolic dysfunctions.
- Soluble dietary fibers are explored for their potential health benefits.
Purpose of the Study:
- To investigate the effects of hydroxypropyl methylcellulose (HPMC) on adipose tissue inflammation and insulin resistance in DIO mice.
- To compare HPMC with insoluble fiber in ameliorating HF diet-induced metabolic changes.
- To assess HPMC's impact on gene expression, glucose/insulin tolerance, and intestinal permeability.
Main Methods:
- DIO C57BL/6J mice were fed a HF diet supplemented with 6% HPMC or 6% microcrystalline cellulose (MCC).
- Gene expression analysis of epididymal adipose tissue was performed using exon microarray and real-time PCR.
- Glucose and insulin tolerance tests and intestinal permeability assays were conducted.
Main Results:
- HPMC supplementation significantly reduced body weight gain and adipose tissue weight.
- HPMC-fed mice showed improved glucose and insulin tolerance, indicated by reduced areas under the curve.
- HPMC decreased HF diet-induced intestinal permeability and downregulated genes related to inflammation, immune response, adipogenesis, and oxidative stress.
Conclusions:
- HPMC consumption ameliorates HF diet effects on obesity-induced insulin resistance.
- HPMC improves adipose tissue inflammatory and immune responses, weight gain, and intestinal permeability.
- HPMC positively influences lipid metabolism, inflammatory disease, and acute phase response pathways.
