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Deoxyribonucleic acid in Nitrobacter carboxysomes.

K Westphal, E Bock, G Cannon

Journal of Bacteriology

|October 1, 1979

Summary

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Carboxysomes from Nitrobacter bacteria contain circular DNA. This double-stranded deoxyribonucleic acid (DNA) was found to be a 14-micron molecule within the icosahedral particles.

Area of Science:

Microbiology
Molecular Biology
Biochemistry

Background:

Carboxysomes are proteinaceous microcompartments found in cyanobacteria and some chemoautotrophic bacteria.
Nitrobacter species are important in the nitrogen cycle, oxidizing nitrite to nitrate.

Purpose of the Study:

To isolate and characterize the genetic material within carboxysomes of Nitrobacter winogradskyi and Nitrobacter agilis.
To determine the physical properties of the DNA found in these bacterial carboxysomes.

Main Methods:

Isolation of carboxysomes from Nitrobacter winogradskyi and Nitrobacter agilis.
Buoyant density centrifugation using ethidium bromide and cesium chloride.
Electron microscopy for DNA visualization and measurement.

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Main Results:

Icosahedral carboxysome particles were successfully isolated.
Double-stranded deoxyribonucleic acid (DNA) was detected within the isolated carboxysomes.
The particle-bound DNA exhibited a buoyant density of rho 25 = 1.701 g/cm3.
Electron microscopy confirmed the DNA as a circular molecule approximately 14 microns in length.

Conclusions:

Nitrobacter carboxysomes encapsulate double-stranded circular DNA.
This finding suggests a potential role for carboxysomes in protecting or organizing genetic material in these bacteria.