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Related Concept Videos

Centrifugation01:05

Centrifugation

Centrifugation is a separation technique based on differences in density or size. It is commonly used to separate solids from aqueous interferents. During centrifugation, the sample is placed in centrifugation tubes and spun at high angular velocity, which allows centrifugal force to act differentially on the different densities or masses of the components. After spinning, the supernatant liquid is decanted. Depending on the specific application, either the pellet or the supernatant is retained...
Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
Subcellular Fractionation01:32

Subcellular Fractionation

The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
Differential centrifugation is...

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Lab-on-a-CD Platform for Generating Multicellular Three-dimensional Spheroids
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Published on: November 7, 2019

Centrifugal microfluidics for cell analysis.

Robert Burger1, Daniel Kirby, Macdara Glynn

  • 1Biomedical Diagnostics Institute, National Centre for Sensor Research, School of Physical Sciences, Dublin City University, Ireland.

Current Opinion in Chemical Biology
|July 13, 2012
PubMed
Summary
This summary is machine-generated.

Centrifugal microfluidic systems offer robust liquid handling for lab-on-a-chip devices. This review focuses on technologies for cell separation and capturing/counting in these systems.

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Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering
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Published on: July 10, 2016

Area of Science:

  • Biotechnology
  • Microfluidics
  • Life Sciences

Background:

  • Centrifugal microfluidic systems have advanced lab-on-a-chip platforms over 20 years.
  • Rotationally controlled actuation is advantageous for cell and particle handling.

Purpose of the Study:

  • To review technologies for cell separation.
  • To review technologies for cell capturing and counting.

Main Methods:

  • Discussion of existing technologies for cell separation on centrifugal microfluidic platforms.
  • Review of methods for cell capturing and counting using centrifugal microfluidics.

Main Results:

  • Centrifugal systems enable high-performance liquid handling.
  • Specific technologies are effective for cell separation.
  • Methods exist for capturing and counting cells within these systems.

Conclusions:

  • Centrifugal microfluidics provides powerful tools for cell handling assays.
  • Further development in separation and counting technologies is highlighted.