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Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...

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Related Experiment Video

Updated: May 20, 2026

Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay
12:49

Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay

Published on: May 25, 2015

How does Lin28 let-7 control development and disease?

James E Thornton1, Richard I Gregory

  • 1Stem Cell Program, Children's Hospital Boston, MA 02115, USA.

Trends in Cell Biology
|July 13, 2012
PubMed
Summary
This summary is machine-generated.

The let-7 microRNA family is crucial for development and acts as a tumor suppressor. The protein Lin28 blocks let-7 production, impacting stem cells, development, and metabolism, offering therapeutic targets.

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The Soft Agar Colony Formation Assay
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Last Updated: May 20, 2026

Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay
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Published on: May 25, 2015

The Soft Agar Colony Formation Assay
08:01

The Soft Agar Colony Formation Assay

Published on: October 27, 2014

Area of Science:

  • Molecular Biology
  • Developmental Biology
  • Genetics

Background:

  • The let-7 microRNA family is a highly conserved regulator of stem cell differentiation and development.
  • let-7 microRNAs function as tumor suppressors.
  • The RNA-binding protein Lin28 is a key developmental regulator known to inhibit let-7 biogenesis.

Purpose of the Study:

  • To review the current understanding of Lin28-mediated control over let-7 maturation.
  • To highlight the role of Lin28 in stem cell biology, development, and glucose metabolism.
  • To discuss the implications of Lin28/let-7 pathway dysregulation in human diseases and potential therapeutic strategies.

Main Methods:

  • Literature review of studies investigating the Lin28-let-7 interaction.
  • Analysis of molecular mechanisms controlling let-7 biogenesis.
  • Examination of the role of Lin28 in various biological processes and disease states.

Main Results:

  • Lin28 directly inhibits the processing of let-7 precursors, thereby controlling let-7 levels.
  • Lin28 plays a critical role in maintaining stem cell pluripotency and regulating developmental timing.
  • Dysregulation of the Lin28-let-7 axis is implicated in cancer, metabolic disorders, and developmental abnormalities.

Conclusions:

  • Lin28 is a central regulator of let-7 maturation, impacting fundamental biological processes.
  • Understanding the Lin28-let-7 pathway offers insights into stem cell biology, development, and metabolism.
  • Targeting the Lin28 pathway presents potential therapeutic avenues for treating cancer and other diseases.