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Extraction of high-quality RNA from rubber tree leaves
Liu-Hong Deng1, Ming-Wu Luo, Chun-Fa Zhang
1Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, China.
Bioscience, Biotechnology, and Biochemistry
|July 13, 2012
Abstract:
A specific technique capable of producing high-quality RNA for rapid amplification of cDNA ends (RACE) was established for challenging tissues: leaves of the rubber tree. Total RNA was extracted by cetyltrimethylammonium bromide (CTAB)-LiCl combined with TRIzol reagent. The isolated RNA was highly intact. With RNA as template, full-length cDNA was obtained (NCBI, AY461413) by RACE.
