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Related Experiment Video

Updated: May 20, 2026

Fluorescence Lifetime Macro Imager for Biomedical Applications

Fluorescence Lifetime Macro Imager for Biomedical Applications

Published on: April 7, 2023

Fluorescence lifetime imaging microscopy using near-infrared contrast agents.

R Nothdurft¹, P Sarder, S Bloch

¹Washington University, School of Medicine, Department of Radiology, Scott Avenue, St. Louis, Missouri 63110, USA.

Journal of Microscopy

|July 14, 2012

Summary

Near-infrared fluorescence lifetime imaging microscopy (FLIM) now images live cells using probes emitting beyond 700 nm. This technique distinguishes cell populations and estimates dye concentrations, bridging in vitro and in vivo imaging.

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Fluorescence Lifetime Macro Imager for Biomedical Applications

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Fluorescence-quenching of a Liposomal-encapsulated Near-infrared Fluorophore as a Tool for In Vivo Optical Imaging

Fluorescence-quenching of a Liposomal-encapsulated Near-infrared Fluorophore as a Tool for In Vivo Optical Imaging

Published on: January 5, 2015

Area of Science:

Biophotonics
Cellular Imaging
Fluorescence Microscopy

Background:

Single-photon fluorescence lifetime imaging microscopy (FLIM) is limited to visible spectrum emissions.
Near-infrared (NIR) probes offer advantages for deep tissue penetration and reduced autofluorescence.

Purpose of the Study:

To explore the feasibility of NIR (emission >700 nm) fluorescent probes for live-cell FLIM.
To establish a method for distinguishing cell populations and estimating dye concentrations using NIR FLIM.

Main Methods:

Utilized a confocal microscope with a 785 nm laser, red-enhanced photomultiplier tube, and time-correlated single photon counting.
Applied NIR fluorescent dyes (cypate and DTTCI) to label live cells.
Acquired and analyzed fluorescence lifetime distributions.

Main Results:

Demonstrated successful lifetime measurements of NIR dyes (cypate, DTTCI) in live cells.
Showcased NIR FLIM's ability to distinguish cell populations based on distinct dye lifetimes.
Enabled estimation of relative dye concentrations within complex cellular microenvironments.

Conclusions:

NIR FLIM is a viable technique for live-cell imaging beyond the visible spectrum.
This method provides a bridge for translating in vitro fluorophore characterization to in vivo small animal imaging.
NIR FLIM offers new possibilities for cellular analysis and in vivo applications.