Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 20, 2026

Synthesis of Decellularized Cartilage Extracellular Matrix Hydrogels
08:34

Synthesis of Decellularized Cartilage Extracellular Matrix Hydrogels

Published on: July 21, 2023

A hydrogel derived from decellularized dermal extracellular matrix.

Matthew T Wolf1, Kerry A Daly, Ellen P Brennan-Pierce

  • 1Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA 15219, USA.

Biomaterials
|July 14, 2012
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Systematic Review of Modeling Platforms for Atrioventricular Valves in Atrioventricular Septal Defects.

Journal of cardiovascular translational research·2026
Same author

PEGylation strategies for enhanced nanoparticle delivery to tumor-associated immune cells.

Bioengineering & translational medicine·2026
Same author

Modeling of contact angle hysteresis for rigid mobile particles: A phase-field lattice Boltzmann approach.

Physical review. E·2026
Same author

Tissue Injury and Biomaterial Treatment Modulate Tumor Growth and Response to Immunotherapy.

bioRxiv : the preprint server for biology·2026
Same author

Discovery of BMS-986458, a Potent and Selective B-Cell Lymphoma 6 Protein Ligand-Directed Degrader, for the Treatment of B-Cell Non-Hodgkin Lymphoma.

Journal of medicinal chemistry·2026
Same author

Quantitative and Qualitative Assessments of the Impacts of a Summer Clinical Immersion Program for Biomedical Engineering Students.

Biomedical engineering education·2026
Same journal

A sonosensitizing hydrogel with tumour-confined stability for intrinsically targeted sonodynamic therapy.

Biomaterials·2026
Same journal

Multidimensional intestinal barrier repair strategies for alleviating inflammatory bowel disease and gut-liver axis-associated metabolic liver disease.

Biomaterials·2026
Same journal

A dual-twisted molecular strategy achieves dramatic quantum-yield enhancement in NIR-II AIEgen for high-performance bioimaging.

Biomaterials·2026
Same journal

An oxygen-glucose co-releasing platform fostering dental pulp regeneration by driving metabolic recovery of stem cells.

Biomaterials·2026
Same journal

Prodrug-decorated 2D hafnium sulfide nanoplatelets as "amplify-and-arrest" platforms for radiosensitization and homologous recombinant inhibition in solid tumor.

Biomaterials·2026
Same journal

In-situ cascade assembled peptide-drug conjugate for the treatment of bladder cancer by enhancing membrane-entry and lysosome destabilization.

Biomaterials·2026
See all related articles

Extracellular matrix (ECM) hydrogels from different tissues show varied properties. Dermal ECM hydrogels offer better mechanical strength and support muscle cell growth, aiding tissue repair.

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Extracellular matrix (ECM) from mammalian tissues serves as a scaffold for tissue repair and reconstruction.
  • ECM hydrogels offer advantages like injectability and inherent bioactivity but their material properties and effects on cell behavior are not well understood.
  • Controlling ECM hydrogel properties is crucial for optimizing tissue regeneration outcomes.

Purpose of the Study:

  • To prepare and characterize ECM hydrogels from decellularized porcine dermis and urinary bladder tissues.
  • To determine the structure, mechanics, and in vitro/in vivo cell response of these ECM hydrogels.
  • To investigate how scaffold tissue source influences ECM hydrogel properties and cell behavior.

Main Methods:

More Related Videos

Development and Characterization of Decellularized Lung Extracellular Matrix Hydrogels
06:12

Development and Characterization of Decellularized Lung Extracellular Matrix Hydrogels

Published on: December 8, 2023

Processing of Human Cardiac Tissue Toward Extracellular Matrix Self-assembling Hydrogel for In Vitro and In Vivo Applications
08:41

Processing of Human Cardiac Tissue Toward Extracellular Matrix Self-assembling Hydrogel for In Vitro and In Vivo Applications

Published on: December 4, 2017

Related Experiment Videos

Last Updated: May 20, 2026

Synthesis of Decellularized Cartilage Extracellular Matrix Hydrogels
08:34

Synthesis of Decellularized Cartilage Extracellular Matrix Hydrogels

Published on: July 21, 2023

Development and Characterization of Decellularized Lung Extracellular Matrix Hydrogels
06:12

Development and Characterization of Decellularized Lung Extracellular Matrix Hydrogels

Published on: December 8, 2023

Processing of Human Cardiac Tissue Toward Extracellular Matrix Self-assembling Hydrogel for In Vitro and In Vivo Applications
08:41

Processing of Human Cardiac Tissue Toward Extracellular Matrix Self-assembling Hydrogel for In Vitro and In Vivo Applications

Published on: December 4, 2017

  • Preparation of ECM hydrogels from decellularized porcine dermal and urinary bladder tissues.
  • Characterization of hydrogel structure, mechanical properties (fiber architecture, integrity, concentration-dependent mechanics).
  • In vitro assessment of C2C12 myoblast fusion, fibroblast infiltration, and contraction; in vivo implantation in rat abdominal wall defects to evaluate host cell infiltration, degradation, and myogenesis.

    • Main Results:

    • Dermal ECM hydrogels exhibited denser fiber architecture and greater mechanical integrity than urinary bladder ECM hydrogels.
    • ECM concentration influenced mechanical properties in a dose-dependent manner for dermal ECM hydrogels.
    • In vitro, dermal ECM hydrogels promoted C2C12 myoblast fusion while reducing fibroblast infiltration and contraction compared to urinary bladder ECM hydrogels.
    • Both hydrogels were infiltrated by host cells (macrophages) in vivo and degraded within 35 days, with urinary bladder ECM hydrogels degrading faster and showing more myogenesis.

    Conclusions:

    • ECM hydrogel properties can be modulated by the choice of tissue source (e.g., dermis vs. urinary bladder).
    • These variations in ECM hydrogel properties significantly impact cellular behavior, including myoblast fusion, fibroblast activity, and host tissue response.
    • The findings highlight the potential for tailoring ECM hydrogels from specific tissues to achieve desired outcomes in regenerative medicine applications.