Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Dynamic bending rigidity of DNA.

L Song1, J M Schurr

  • 1Department of Chemistry, University of Washington, Seattle 98195.

Biopolymers
|January 1, 1990
PubMed
Summary
This summary is machine-generated.

Transient electric dichroism reveals DNA bending dynamics. New theory explains relaxation times and persistence length, suggesting dynamic bending rigidity exceeds static measurements.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dynamic light-scattering studies of internal motions in DNA. III. Evidence for titratable joints associated with bound polycations.

Biopolymers·2020
Same author

Clinical utility of quantitative dual-energy CT iodine maps and CT morphological features in distinguishing small-cell from non-small-cell lung cancer.

Clinical radiology·2019
Same author

[Impact of coronary artery lesion calcification on the long-term outcome of patients with coronary heart disease after percutaneous coronary intervention].

Zhonghua xin xue guan bing za zhi·2019
Same author

Effect of storage temperature on bacterial diversity in chicken skin.

Journal of applied microbiology·2018
Same author

[Effect of hnRNP K and its interaction with HPV16 on cervical intraepithelial neoplasia].

Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi·2018
Same author

[Preparation and evaluation of blank and doxorubicin loaded poly (acrylic acid) microspheres for embolization].

Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences·2018
Same journal

Untreated Rosehip Powder/Poly(Lactic Acid)/Poly(3-Hydroxybutyrate-Co-4-Hydroxybutyrate) Electrospun Mats for Wound Dressing Applications.

Biopolymers·2026
Same journal

Synthesis, Characterization, and Antidiabetic Evaluation of Sequence-Modified Liraglutide Analogs in a Drosophila melanogaster Model.

Biopolymers·2026
Same journal

Fabrication of an Antibacterial Alginate/Chitosan Hydrogel Dressing Loaded With CuO Nanoparticles for Wound Dressing Applications.

Biopolymers·2026
Same journal

Effect of Chitosan-Alginate Polyelectrolyte Complex Formation and Multilayer Polymer Configuration on the Characteristics of 3D-Printed Metronidazole-Loaded Periodontal Films.

Biopolymers·2026
Same journal

Phenolic Grafting of Oxidized Cellulose Nanofibers Using Ferulic Acid: Structural and Antioxidant Analysis Toward Bioactive Nanomaterials.

Biopolymers·2026
Same journal

Detection of a Target Nucleic Acid by Ligation-Assisted Fluorescence Enhancement of a Peptide Nucleic Acid (PNA) Twin Probe via Disulfide Binding.

Biopolymers·2026
See all related articles

Area of Science:

  • Biophysics
  • Molecular Biology
  • Polymer Physics

Background:

  • Previous studies reported rapidly relaxing components in DNA transient electric dichroism.
  • These components were observed in DNA restriction fragments.

Purpose of the Study:

  • Analyze DNA bending dynamics using a new normal mode theory.
  • Assign relaxation components to bending modes.
  • Determine the dynamic bending rigidity and persistence length of DNA fragments.

Main Methods:

  • Analysis of transient electric dichroism decay curves.
  • Application of normal mode theory for weakly bending rods.
  • Comparison of experimental data with theoretical predictions.

Main Results:

Related Experiment Videos

  • The longest bending relaxation times for DNA fragments (95-250 base pairs) match theoretical curves.
  • A dynamic persistence length (Pd) of 2100 A was calculated.
  • Relative amplitudes of fast and slow relaxation components support a large dynamic persistence length.
  • Enhanced fast component amplitude in high electric fields attributed to steady-state bending.

Conclusions:

  • The study provides a theoretical framework for understanding DNA bending dynamics.
  • Dynamic bending rigidity of DNA is significantly higher than static measurements suggest.
  • Electric fields can induce steady-state bending in DNA molecules.