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Related Experiment Video

Updated: May 20, 2026

Subcutaneous Angiotensin II Infusion using Osmotic Pumps Induces Aortic Aneurysms in Mice

Subcutaneous Angiotensin II Infusion using Osmotic Pumps Induces Aortic Aneurysms in Mice

Published on: September 28, 2015

Annexin A1 modulates macula densa function by inhibiting cyclooxygenase 2.

S Seidel¹, H Neymeyer, T Kahl

¹Department of Anatomy, Charité-Universitätsmedizin Berlin, Germany.

American Journal of Physiology. Renal Physiology

|July 14, 2012

Summary

Annexin A1 (ANXA1) inhibits cyclooxygenase 2 (COX-2) in kidney macula densa cells. This finding suggests ANXA1 is a novel regulator of renal function by controlling prostaglandin synthesis.

Related Experiment Videos

Last Updated: May 20, 2026

Subcutaneous Angiotensin II Infusion using Osmotic Pumps Induces Aortic Aneurysms in Mice

Subcutaneous Angiotensin II Infusion using Osmotic Pumps Induces Aortic Aneurysms in Mice

Published on: September 28, 2015

Area of Science:

Nephrology
Molecular Biology
Inflammation Research

Background:

Annexin A1 (ANXA1) is an anti-inflammatory protein known to interact with formyl peptide receptors (Fpr).
ANXA1 is present in juxtaglomerular apparatus cells, but its role in regulating cyclooxygenase 2 (COX-2) in the macula densa is unclear.

Purpose of the Study:

To investigate the hypothesis that ANXA1 regulates COX-2 biosynthesis in the kidney macula densa.
To elucidate the molecular mechanisms underlying ANXA1's effect on COX-2 expression.

Main Methods:

Immunohistochemical colocalization of ANXA1 and COX-2 in rat kidneys.
Analysis of ANXA1 and COX-2 expression in ANXA1-deficient mice.
In vitro studies using cultured macula densa cells with ANXA1 transfection and dexamethasone treatment.
Pharmacological inhibition of the ANXA1 receptor Fpr1.

Main Results:

High colocalization of ANXA1 and COX-2 (95%) in rat macula densa.
Furosemide treatment increased ANXA1 and COX-2 expression with maintained colocalization.
ANXA1 deficiency in mice led to increased COX-2 and renin expression.
ANXA1 transfection and dexamethasone treatment downregulated COX-2 mRNA in cultured cells.
Fpr1 inhibition blunted dexamethasone's effect on COX-2.

Conclusions:

ANXA1 exerts an inhibitory effect on COX-2 expression in the macula densa.
ANXA1 may act as an intrinsic modulator of renal juxtaglomerular regulation through inhibition of prostaglandin E2 synthesis.